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Wnt4 expression is up-regulated in XY Sox9 mutant gonads. (A,B) RNAscope® detection of Wnt4 transcripts (red) together with immunodetection of the interstitial and stromal cell marker NR2F2 (green) in E12.5 XX Control gonad (A), XY Control gonad (B), and XY Sox9cKO gonad (C). Nuclei labeled with DAPI are shown in grey. Magnification is the same in all panels. Scale bar = 100 μm. Gonads are outlined with broken white lines.
