Figure 5.

Co-immunoprecipitation of AQP8 and HPV L1 proteins in human sperm cells. (A) The sperm cell lysates of HPV16 infected subjects were subjected to co-immunoprecipitation with a HPV L1 specific antibody; protein expression of both AQP8 and L1 was detected by immunoblotting with anti-AQP8 and with anti-HPV antibodies as indicated in Materials and methods. (B) As negative control, HPV-negative sperm cells lysates were incubated with the anti-HPV antibody. Major bands of AQP8 and L1 proteins were shown. OS, original sample; FT, flow-through; W, wash; E, elution, PC, positive control.