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. 2020 May 14;9(5):1220. doi: 10.3390/cells9051220

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Xylosyl extension of O-Glc glycans enhances the cell surface expression of NOTCH1 or NOTCH2 overexpressed in HEK293T cells. (A) The cell surface expression of transfected full length NOTCH1 in the wild type and XYLTs’ KO clones of HEK293T cells analyzed by flow cytometry. Co-transfected GFP-positive cells were gated. (B) Mean fluorescence intensity from (A). Plots are from four independent experiments (n = 4). Bar graphs show the average ± SEM. (C) Cell surface expression of transfected full length NOTCH2 in the wild type and XYLTs’ KO clones of HEK293T cells analyzed by flow cytometry. Co-transfected GFP-positive cells were gated. (D) Mean fluorescence intensity from (C). Plots are from three independent experiments (n = 3). Bar graphs show the average ± SEM. The cell numbers on the vertical axis of the graphs in (A,C) are normalized with the mode value. *, p < 0.05.