Figure 5.
Hemisphere-specific PNN density changes are not conserved in Mecp2Het after maternal behavior. (A) Combined hemispheric analysis of PNN density of SS1 did not reveal significant changes between naïve Het (NH, n = 123 images) and surrogate Het (SH, n = 162 images) (Mann-Whitney test, p > 0.05). (B) Analysis of PNN density between hemispheres of SS1 revealed no significant difference between conditions or within hemispheres of naïve or surrogate Het (n = 54–82 images; Kruskal-Wallis followed by Dunn’s test, p > 0.05). (C) Analysis of SS1 area in both hemispheres revealed no significant changes after maternal learning (NH: n = 123 images; SH: n = 162 images; Mann-Whitney test, p > 0.05). (D) Area analysis by hemispheres reveal that left hemisphere of SH (SH-L) was significantly larger than the right hemisphere of SH (SH-R). This hemispheric area bias was absent in NH (n = 54–82 images; Kruskal-Wallis followed by Dunn’s test, **p < 0.01). n.s. = not significant. 5 mice per condition.