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. 2020 May 23;10(15):6898–6914. doi: 10.7150/thno.42347

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Transcriptome analysis of MESP1-mTomato⁺ cardiovascular progenitor cells. (A) Flow chart of MESP1-mTomato cell gene expression analysis. (B) PCA graph showing that hESCs, MESP1-mTomato⁺ cells at day 3 and 5 (MT_day3, MT_day5), and MESP1-mTomato^- cells (MT_neg) were separated from each other. (C) Representative gene expression patterns in day 3 and day 5 MESP1⁺ cells. The number of genes in each category, representative GO terms and enrichment P values were listed on the right side. (D) Heatmaps showing that many key regulators of cardiovascular development were differentially expressed in day 3 or day 5 MESP1⁺ cells, including Wnt pathway members, heart development genes, endothelial genes, hematopoietic cell genes and skeletal development genes. (E) GSEA plot of selective biological process enriched in MESP1⁺ or MESPI^- cells, day 3 or day 5 MESP1 cells.