FIG 8.
sRNA 179 modulates the Rsm system by increasing RsmY transcription. (A) The indicated strains carrying a PexsD-lacZ reporter were transformed with either the vector control (pJN), the Hfq expression vector, or sRNA 0161 and 179 expression vectors. Strains were cultured under inducing conditions for T3SS gene expression supplemented with 0.05% or 0.2% arabinose to induce Hfq or sRNA expression, respectively, and assayed for PexsD-lacZ reporter activity as reported in Miller units. The reported values with the standard error represent the average of at least three experiments. *, P < 0.05. (B) WT carrying either a PrsmY-lacZ or PrsmY-lacZ transcriptional reporter and the indicted expression vectors were assayed for reporter activity as in panel A. *, P < 0.005. (C and D) Comparison of RsmY (C) and RsmZ (D) levels in RNA samples harvested from the indicated strains. RNA samples were quantified by qRT-PCR and normalized to the rimM housekeeping gene. The reported values are relative to each strain carrying the vector control.