Fig. 2.

The inability of PF-4708671 and BQR to inhibit PEDV replication. Vero cells infected with HXLV strain of PEDV (0.5 MOI) were incubated in the absence or presence of the indicated concentrations of BQR (A) or PF-4708671 (B & C) for 12 h. The cell lysates were analyzed for the expression of the S and N proteins (A & B) or analyzed for the levels of protein phosphorylation (C) by Western blot. (D & E) Effect of BQR (20 μM) and PF-4708671 (20 μM) on Vero cell proliferation. Vero cells seeded in 96-well plates were left uninfected or infected with PEDV virus (0.5 MOI) and then incubated in the absence or presence of BQR (20 μM) (D) and PF-4708671 (20 μM) (E). After incubation for 12 h, the cells were analyzed for proliferation by using a CellTiter-Glo kit. Data are the mean ± SD of the triplicate from one representative of three independent experiments with similar results and statistically analyzed by a Student's t-test.