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[Preprint]. 2022 May 18:2020.06.02.20120477. Originally published 2020 Jun 4. [Version 2] doi: 10.1101/2020.06.02.20120477

Serological assessment of SARS-CoV-2 exposure in northern Sweden by the use of at-home sampling to meet geographical challenges in rural regions

Julia Wigren Byström, Linnea Vikström, Ebba Rosendal, Remigius Gröning, Yong-Dae Gwon, Emma Nilsson, Atin Sharma, Akbar Espaillat, Leo Hanke, Gerald McInerney, Andrea Puhar, Felipe Cava, Gunilla B Karlsson Hedestam, Therese Thunberg, Tor Monsen, Fredrik Elgh, Magnus Evander, Anders Johansson, Anna K Överby, Clas Ahlm, Johan Normark, Mattias NE Forsell
PMCID: PMC7302301  PMID: 32577692

ABSTRACT

The current SARS-CoV-2 pandemic has highlighted a need for easy and safe blood sampling in combination with accurate serological methodology. Venipuncture is usually performed by trained staff at health care centers. Long travel distances may introduce a bias of testing towards relatively large communities with close access to health care centers. Rural regions may thus be overlooked. Here, we demonstrate a sensitive method to measure antibodies to the S-protein of SARS-CoV-2. We adapted and optimized this assay for clinical use together with capillary blood sampling to meet the geographical challenges of serosurveillance. Finally, we tested remote at-home capillary blood sampling together with centralized assessment of S-specific IgG in a rural region of northern Scandinavia that encompasses 55,185 sq kilometers. We conclude that serological assessment from capillary blood sampling gives comparable results as analysis of venous blood. Importantly, at-home sampling enabled citizens living in remote rural areas access to centralized and sensitive laboratory antibody tests.

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