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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Pain. 2020 Jul;161(7):1636–1649. doi: 10.1097/j.pain.0000000000001846

Table 2.

Primer sequences used for single-cell semi-quantitative PCR.

GAPDH- Fwd ACTCAGTCCCCCACCACACT
GAPDH- Rev GGTACATGACAAGGTGCGGC
LYS- Fwd GCCATATCGGCTCGCAAATC
LYS- Rev AACGAATGCCGAAACCTCCTC
OPRM1- Fwd AGTGTGTTAGTTCCATCATCATGTC
OPRM1- Rev TCTGATCATCCTGTCCTACCTG
OPRD1- Fwd CGGGACCTGTGGCTCTACAA
OPRD1- Rev CCCCCAACTCCGAAAGTCCG
OPRK1- Fwd AATTCAGGGAGCACACTGGC
OPRK1- Rev AAATTGCCTTAAGGAAGCTGGGT
OPRL1- Fwd GGAAGTGATGGCTGTCTCGC
OPRL1- Rev GAGCCTCCCCAGAACAGTCC
TRPV1- Fwd TTGGGGGTGTTGGTGTTTCCT
TRPV1- Rev AGCAGGACTGGGGTTCCTAGA

Forward (Fwd) and reverse (Rev) primers for glyceraldehyde 3-phosphate dehydrogenase (GAPDH), lysis protein (LYS), opioid receptor mu 1 (OPRM1), opioid receptor delta 1 (ORPD1), opioid receptor kappa (OPRK1), opioid related nociceptin receptor 1 (OPRL1), and transient receptor potential cation channel subfamily V member 1 (TRPV1).