Abstract
Refractory hypoxemia is the main symptom of acute respiratory distress syndrome (ARDS). Low tidal volume ventilation is routinely applied in clinical practice to correct hypoxemia, which aims to prevent ventilator-induced lung injury. However, this ventilation strategy inevitably leads to hypercapnia. Our previous study demonstrated that hypercapnia aggravated cognitive impairment in hypoxemic rats; however, the underlying mechanism remains unclear. The aim of the present study was to investigate whether hypercapnia exacerbates the blood-brain barrier (BBB) disruption through inducing interleukin (IL)-1β overproduction in the blood of hypoxemic rats. The BBB permeability in a rat model of hypercapnia/hypoxemia was evaluated. The levels of IL-1β in the blood of rats and human whole-blood cultures were assessed. The expression of IL-1 receptor 1 (IL-1R1), phosphorylated IL-1R1-associated kinase (p-IRAK-1) and tight junctional proteins in cerebral vascular endothelial cells was examined in vitro and in vivo. In addition, IL-1Ra, an IL-1 receptor antagonist, was used to determine whether hypercapnia affects tight junctional protein expression in hypoxic cerebral vascular endothelial cells through inducing IL-1β overproduction. It was observed that hypercapnia alone did not disrupt the BBB, but aggravated the damage to the BBB integrity in hypoxemic rats. Hypercapnia increased IL-1β expression in the blood of hypoxemic rats as well as in hypoxic human whole-blood cultures. IL-1R1 and p-IRAK-1 expression was increased, while that of tight junctional proteins was reduced by hypercapnia in hypoxemic cerebral vascular endothelial cells in vitro and in vivo. Additionally, the expression of tight junctional proteins was markedly increased following treatment with IL-1Ra. These results suggest that hypercapnia-induced IL-1β overproduction in the hypoxemic blood may decrease tight junctional protein expression in cerebrovascular endothelial cells via the IL-1R1/p-IRAK-1 pathway, further disrupting BBB integrity, and eventually resulting in increased BBB permeability.
Keywords: acute respiratory distress syndrome, blood-brain barrier, interleukin-1β, hypercapnia, hypoxemia
Introduction
Acute respiratory distress syndrome (ARDS) is one of the major critical diseases encountered in intensive care units (1). The main symptom of ARDS is refractory hypoxemia. Low tidal volume ventilation is routinely applied in clinical practice to correct hypoxemia, which aims to reduce the risk of ventilator-induced lung injury (2). However, this ventilation strategy inevitably leads to hypercapnia, which is referred to as 'permissive' hypercapnia (3). Permissive hypercapnia may contribute to an improved outcome in terms of pulmonary function (4,5), but its effects on cerebral function, such as cognitive function, have not been extensively investigated. Indeed, our previous study demonstrated that permissive hypercapnia aggravated cognitive impairment in hypoxemic rats (6); however, the underlying mechanism remains unclear.
It has been reported that there is a close association between disruption of the blood-brain barrier (BBB) and cognitive impairment (7-10). BBB disruption, particularly in the region of the hippocampus, may lead to cognitive dysfunction (11,12). A recent study demonstrated that BBB breakdown played a predictive role in human cognitive disorders, particularly at the early stages (13). Moreover, Wang et al reported that hyper-permeability of the BBB resulted in cognitive impairment in a rat model of splenectomy (14). However, it remains unknown whether hypercapnia exerts any effects on BBB disruption in hypoxemic rats.
Tight junctions between cerebrovascular endothelial cells play a key role in maintaining the integrity of the BBB. Tight junctional proteins, including zonula occludens (ZO)-1, occludin and claudin-5, are involved in the determination of BBB permeability (15-17). Additionally, previous studies suggested that peripheral inflammation is crucial in the process of BBB disruption (18,19). Interleukin (IL)-1β, one of the major pro‑inflammatory factors, may also be involved in the disruption of the BBB. Wang et al revealed that IL-1β induced BBB breakdown through suppressing sonic hedgehog expression in astrocytes. They also observed that IL-1β promoted the expression of other inflammatory factors, thereby augmenting inflammation and aggravating BBB disruption (20). It remains to be determined whether hypercapnia would further increase BBB permeability through upregulating IL-1β expression in the blood of hypoxemic rats.
The aim of the present study was to determine whether hypercapnia can exacerbate BBB disruption through inducing IL-1β overproduction in the blood of hypoxemic adult rats, and whether this effect is mediated by triggering the expression of IL-1 receptor 1 (IL-1R1) in cerebrovascular endothelial cells, decreasing the expression of tight junctional proteins, and ultimately increasing BBB permeability.
Materials and methods
Animals and treatment
Male adult Sprague-Dawley rats (n=144), aged ~3 months and weighing 220-250 g, were included in present study. All efforts were made to mini-mize the number of animals used in the experiments. The rats were fed standard chow and water, and were housed under standard experimental conditions (temperature, 20-25°C; humidity, 50-70%) under a 12-h light/dark cycle. All rats were fasted with access to water overnight prior to the experiments. The rat model of hypercapnia/hypoxemia was established as described in our previous study (6). To minimize suffering and distress, the rats were anesthetized with 2% pentobarbital sodium (30 mg/kg by intraperitoneal injection) followed by mechanical ventilation. The tidal volume (9 ml/kg body weight), respiratory rate (45 breaths/min), and inspiratory:expiratory ratio (1:1) were fixed. The rats were randomly divided into four groups according to different concentrations of O2 and CO2 as follows: Sham group (exposed to air), hypercapnia group (exposed to 5% CO2), hypoxemia group (exposed to 16% O2), and hypercapnia + hypoxemia group (HH group; exposed to 16% O2 mixed with 5% CO2). These concentrations (16% O2 and 5% CO2) were used to maintain the PO2 of arterial blood at ~60 mmHg and the pH at 7.20-7.25. The right femoral artery was cannulated to collect arterial blood samples. The PO2, PCO2 and pH of the arterial blood samples were immediately measured by a Blood Gas/Electrolyte Analyzer (Model 5700; Werfen Corporation).
The rats that were used for western blotting and immu-nofluorescence staining were not subjected to invasive manipulation apart from ventilation. All animals were ventilated for 3 h, after which time they were euthanized by intraperitoneal injection of pentobarbital sodium (150 mg/kg).
Assessment of BBB permeability
At 3 h after ventilation, 2% Evans blue (EB) solution (4 ml/kg) was injected through the caudal vein. After 1 h, the rats were perfused transcardially with normal saline to remove the intravascular dye, and 4% paraformaldehyde was used to perfuse the brain. The brains were harvested and incubated in formamide (1 ml/100 mg) at 60°C for 24 h. The supernatant was separated after centrifugation at 12,000 x g at 4°C for 20 min. The optical density (OD) values were measured at 620 nm using a spectrophotometer (Multiskan FC IVD; Thermo Fisher Scientific, Inc.).
Human whole‑blood cultures and ethics statement
Whole blood was donated by 4 healthy male volunteers (mean age ± standard deviation, 35±9.4 years). None of the volunteers had a history of cancer, hematological disorders, infections, autoimmune diseases, transplantation, or use of immunosuppressive drugs. The whole blood was cultured as reported previously (21). Briefly, 2 ml of whole blood were collected from each volunteer. Subsequently, the whole-blood samples were mixed with 18 ml RPMI-1640 medium (Invitrogen; Thermo Fisher Scientific, Inc., cat. no. 8118329), which was supplemented with 10% human serum (Gemini Bio-Products; cat. no. H91S001) and 1% penicillin-streptomycin solution (Aladdin Biochemical Technology Co., Ltd.; cat. no. P113150). The blood samples were randomly divided into four groups as follows: Control, high concentration of CO2 (HC), hypoxia, and hypoxia + + HC groups. The control group was exposed to 5% CO2 + 20% O2. The HC group was exposed to 10% CO2 + 20% O2. The hypoxia group was exposed to 5% CO2 + 0.2% O2. The hypoxia + HC group was exposed to 10% CO2 + 0.2% O2. These concentrations (0.2% O2 and 10% CO2) were used to maintain the PO2 of the medium at ~60 mmHg and the pH at 7.20-7.25. The PO2, PCO2 and pH of the medium were measured by a Blood Gas/Electrolyte Analyzer (Model 5700; Werfen Corporation).
IL‑1β evaluation by ELISA in vitro and in vivo
The levels of IL-1β were evaluated using ELISA kits (applied to human blood cultures, cat. no. ab100562; applied to rat blood, cat. no. ab100768; Abcam) following the manufacturers' instructions. Briefly, the samples and standards were added to the plate wells coated by IL-1β antibodies labeled with horseradish peroxidase (HRP). Subsequently, a TMB substrate solution was pipetted to the wells. Then, stop buffer was added, and the OD was measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-1β in the samples was determined by comparing the OD of the samples to the standard curve.
Primary cultures of rat brain capillary endothelial cells (RBECs)
Primary cultures of RBECs were prepared as previously described (22–24). Briefly, the gray matter was isolated from the brain of 3-week-old Sprague-Dawley rats. The gray matter was minced into tiny particles, digested with DMEM containing collagenase type 2 (1 mg/ml), gentamycin (50 μg/ml), and 300 μl DNase (15 μg/ml) at 37°C for 1.5 h, then neutralized with bovine serum albumin (Calbiochem-Novabiochem Corp.), and finally centrifuged at 1,000 x g at 4°C for 20 min. The cells were further digested in DMEM containing collagenase-dispase (1 mg/ml) and DNase (6.7 μg/ml) for 1 h at 37°C. Microvessel endothelial cell clusters were separated on a 33% continuous Percoll gradient, then collected and washed twice with DMEM before plating into collagen type IV‑ and fibronectin‑coated dishes. RBECs were cultured in DMEM/F12 supplemented with 10% plasma-derived serum, heparin (100 μg/ml), basic fibroblast growth factor (1.5 ng/ml), transferrin (5 μg/ml), insulin (5 μg/ml), sodium selenite (5 ng/ml), puromycin (4 μg/ml) and gentamycin (50 μg/ml) (RBEC medium I) at 37°C in a humidified incubator with 5% CO2/95% air for 2 days. On the following day, RBECs were cultured in RBEC medium II (RBEC medium I without puromycin). The medium was changed every other day from the 4th day onwards. The RBECs were randomly divided into four groups as follows: Control, IL-1β, IL-1β + IL-1 receptor antagonist (IL-1Ra), and IL-1Ra groups. The control group was treated with 0.01 M PBS. The IL-1β group was treated with IL-1β (40 ng/ml; MedChemExpress; cat. no. HY-P7028). The IL-1β + IL-1Ra group was treated with IL-1β (40 ng/ml) and IL-1Ra (40 ng/ml; MedChemExpress; cat. no. HY-P7029). The IL-1Ra group was treated with IL-1Ra (40 ng/ml) (25).
Western blotting
Total proteins from the hippocampal tissue samples and RBECs (n=4 for each group) were extracted using a Total Protein Extraction kit (BestBio; cat. no. BB-3101-100T). Equal amounts (40 μg) of proteins from each sample were separated in a 10% SDS-PAGE gel and transferred to PVDF membranes (EMD Millipore), which were then blocked with 5% non-fat milk for 1 h at room temperature. Subsequently, the membranes were incubated overnight at 4°C with primary antibodies against the following target proteins: IL-1R1 (1:1,000, Abcam; cat. no. ab106278), ZO-1 (1:1,000; Invitrogen; Thermo Fisher Scientific, Inc.; cat. no. SL258826), occludin (1:1,000; Abcam; cat. no. Ab216327), claudin-5 (1:1,000; Abbkine Scientific Co., Ltd.; cat. no. Abp50990), t‑IRAK‑1 (1:1,000; Abcam; cat. no. ab238) and p-IRAK-1 (1:1,000; Abcam; cat. no. ab218130). On the following day, HRP-labeled goat anti-rabbit antibody (1:3,000; Cell Signaling Technology, Inc.; cat. no. 7074S) was added and the membranes were incubated for 2 h at 4°C. The immunoblots were visualized using a chemiluminescence kit (Bioworld Technology, Inc.; cat. no. AC36131), and detected by an imaging densitometer (ImageQuant LAS 500, GE Healthcare Bio-Sciences AB). The gray value was quantified using FluorChem 8900 software (version 4.0.1, Alpha Innotech Corporation). β-actin was used as the control. The relative density was calculated through dividing the gray value of β-actin by that of the target protein. Both the target protein and β-actin were from the same lane of the same membrane.
Double immunofluorescence labeling
In vivo, the rats were anesthetized with 2% pentobarbital sodium (30 mg/kg by intraperitoneal injection) and transcardially perfused with saline and 4% paraformaldehyde at 3 h after ventilation. The brains were harvested and post‑fixed overnight at 4°C in 4% paraformaldehyde. Subsequently, the tissue samples were dehydrated in graded sucrose and cut into 10-μm sections. The sections were blocked in 5% normal donkey serum (Abbkine Co., Ltd. cat. no. BMS0140) for 0.5 h at room temperature. In vitro, the coverslips with adherent RBECs were fixed with 4% paraformaldehyde for 20 min at room temperature at 24 h after treatment. The coverslips were blocked in 5% normal donkey serum for 0.5 h at room temperature sequentially.
Subsequently, the sections/coverslips were incubated overnight at 4°C with the following primary antibodies: IL‑1R1 (1:100, Abcam; cat. no. ab106278), ZO-1 (1:100; Invitrogen; Thermo Fisher Scientific Inc.; cat. no. SL258826), occludin (1:100; Abcam; cat. no. Ab216327), claudin-5 (1:100; Abbkine Scientific Co., Ltd.; cat. no. Abp50990), and CD31 (1:100; Abcam; cat. no. Ab24590). The sections/coverslips were washed on the following day, the secondary antibodies Alexa Fluor® 549 goat anti-rabbit IgG (H + L) (1:100; Invitrogen; Thermo Fisher Scientific Inc.; cat. no. ATRJN1301) and Alexa Fluor® 488 goat anti-mouse IgG (1:100; Invitrogen; Thermo Fisher Scientific Inc.; cat. no. ATRMR2301) were added, and the sections/coverslips were incubated for 1 h at room temperature. Finally, the sections/coverslips were mounted using fluorescent mounting medium with DAPI (Sigma-Aldrich; Merck KGaA; cat. no. SLBW4468) and examined using a fluorescence microscope (DP73, Olympus Corporation). At a magnification of x400. Four fields of view per section/coverslip were examined in each group.
Statistical analysis
The statistical analysis was performed using SPSS v.19.0 (IBM Corp.). All values are expressed as mean ± standard deviation. Student's t-test was used to analyze the data of two-group univariate-factor measurements. One-way analysis of variance (ANOVA) was used to analyze the data of 3 or more group univariate-factor measurements followed by Tukey's post hoc test. Repeated measures ANOVA was used to analyze the repeated measurement data. Factorial ANOVA was used for the interaction effects. When an interaction was examined, simple effects analyses were evaluated. P<0.05 was considered to indicate statistically significant differences.
Results
PO2, PCO2 and pH levels in the arterial blood of the rats
The PO2 levels in the arterial blood were maintained at ~60 mmHg in the HH and hypoxemia groups, and were significantly decreased compared with those in the Sham group (HH vs. Sham: P<0.01; hypoxemia vs. Sham: P<0.01) and the hypercapnia group (HH vs. hypercapnia: P<0.01; hypoxemia vs. hypercapnia: P<0.01). The PO2 levels of the HH group were not significantly different when compared with those of the hypoxemia group (P>0.05; Fig. 1A-a). The PCO2 levels in the arterial blood were maintained at 60-70 mmHg in the HH and hypercapnia groups, and were significantly higher compared with those in the Sham group (HH vs. Sham: P<0.01; hypercapnia vs. Sham: P<0.01) and the hypoxemia group (HH vs. hypoxemia: P<0.01; hypercapnia vs. hypoxemia: P<0.01). There was no significant difference between the HH and hypercapnia groups in the PCO2 levels (P>0.05; Fig. 1A-b). The pH of the arterial blood was maintained at 7.20-7.25 in the HH and hypercapnia groups, and was significantly lower compared with that in the Sham group (HH vs. Sham: P<0.01; hypercapnia vs. Sham: P<0.01) and the hypoxemia group (HH vs. hypoxemia: P<0.01; hypercapnia vs. hypoxemia: P<0.01). There was no significant difference in the pH levels between the HH and hypercapnia groups (P>0.05; Fig. 1A-c).
PO2, PCO2 and pH levels in the whole‑blood culture medium
The PO2 levels were maintained at ~60 mmHg in the hypoxia + HC and hypoxia groups, and were significantly decreased compared with those in the control group (hypoxia + HC vs. control: P<0.01; hypoxia vs. control: P<0.01) and the HC group (hypoxia + HC vs. HC: P<0.01; hypoxia vs. HC: P<0.01). The PO2 levels of the hypoxia + HC group were not significantly different when compared with those of the hypoxia group (P>0.05; Fig. 1B-a). The PCO2 levels were maintained at ~80 mmHg in the hypoxia + HC and HC groups, and were significantly higher compared with those in the control group (hypoxia + HC vs. control: P<0.01; HC vs. control: P<0.01) and the hypoxia group (hypoxia + HC vs. hypoxia: P<0.01; HC vs. hypoxia: P<0.01). There was no significant difference in the PCO2 levels between the hypoxia + HC and HC groups (P>0.05; Fig. 1B-b). The pH was maintained at 7.20-7.25 in the hypoxia + HC and HC groups, and was significantly decreased compared with that in the control group (hypoxia + HC vs. control: P<0.01; HC vs. control: P<0.01) and the hypoxia group (hypoxia + HC vs. hypoxia: P<0.01; HC vs. hypoxia: P<0.01). There was no significant difference in the pH levels between the hypoxia + HC and HC groups (P>0.05; Fig. 1B-c).
Hypercapnia increases IL‑1β expression in the blood of hypoxemic rats
An interaction effect was observed between hypoxia treatment and hypercapnia treatment (P<0.01; Fig. 2A). Simple effects analyses found increased IL-1β expression in the hypoxemia group (P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The HH group exhibited the highest expression levels of IL-1β when compared with the hypoxemia group (P<0.01) and the hypercapnia group (P<0.01; Fig. 2B).
Treatment with 10% CO2 increases IL‑1β expression in hypoxic whole‑blood cultures
An interaction effect was observed between 10% CO2 treatment and 0.2% O2 treatment (P<0.01; Fig. 2C). Simple effects analyses found increased IL-1β expression in the hypoxia group (P<0.01), but not in the HC group (P>0.05) compared with the control group. The hypoxia + HC group exhibited the highest expression levels of IL-1β compared with the hypoxia group (P<0.01) and the HC group (P<0.01; Fig. 2D).
Hypercapnia increases IL‑1R1 expression in the cerebrovascular endothelial cells of hypoxemic rats
An interaction effect was observed between hypoxia treatment and hypercapnia treatment (P<0.05; Fig. 3B). Simple effects analyses found increased IL-1R1 expression in the hypoxemia group (P<0.05), but not in the hypercapnia group (P>0.05) when compared with the Sham group. The expression levels of IL-1R1 were the highest in the HH group compared with those in the hypoxemia group (P<0.01) and the hypercapnia group (P<0.01; Fig. 3C). Double immunofluorescence was used to examine IL-1R1 expression in cerebrovascular endothelial cells. Enhanced IL‑1R1 immunofluorescence was observed in the hypoxemia group, but not in the hypercapnia group compared with the Sham group. The HH group exhibited the most intense IL-1R1 fluorescence compared with the hypoxemia and hypercapnia groups (Fig. 3D).
IL‑1β treatment increases IL‑1R1 and p‑IRAK‑1 expression in RBECs
IL-1R1 expression was increased in the IL-1β group compared with that in the control group (P<0.01; Fig. 4B). Double immunofluorescence was used to examine IL-1R1 expression in RBECs. Enhanced IL‑1R1 immunofluorescence was observed in the IL-1β group compared with that in the Sham group (Fig. 4C). Increased p-IRAK-1 expression was observed in the IL-1β group compared with that in the control group (P<0.01). The protein expression of p‑IRAK‑1 was significantly suppressed with IL-1Ra pretreatment (P<0.05; Fig. 4E).
Hypercapnia decreases tight junctional protein expression in the cerebrovascular endothelial cells of hypoxemic rats
Significant interaction effects were observed between hypoxia treatment and hypercapnia treatment (ZO-1: P<0.05; occludin: P<0.05; and claudin-5: P<0.05; Fig. 5B-D). Simple effects analyses found decreased expression of tight junctional proteins in the hypoxemia group (ZO-1: P<0.01; occludin: P<0.01; and claudin-5: P<0.05), but not in the hypercapnia group (ZO-1: P>0.05; occludin: P>0.05; and claudin-5: P>0.05) compared with the Sham group. The HH group exhibited the lowest expression levels of tight junctional proteins in comparison with the hypoxemia group (ZO-1: P<0.01; occludin: P<0.05; and claudin-5: P<0.05) and the hypercapnia group (ZO-1: P<0.01; occludin: P<0.01; and claudin-5: P<0.01; Fig. 5E). Double immunofluorescence was used to examine tight junctional protein expression in cerebrovascular endothelial cells (Figs. S1-S3). Reduced ZO-1, occludin and claudin-5 immunofluorescence was observed in the hypoxemia group, but not in the hypercapnia group, when compared with the Sham group. The HH group exhibited the weakest ZO-1, occludin and claudin‑5 fluorescence compared with the hypoxemia and hypercapnia groups.
IL‑1β treatment decreases tight junctional protein expression in RBECs
The IL-1β group exhibited decreased tight junctional protein expression compared with the control group (ZO-1: P<0.01; occludin: P<0.01; and claudin-5: P<0.01). The tight junctional protein expression was significantly upregulated with IL-1Ra pretreatment (ZO-1: P<0.01; occludin: P<0.01; and claudin-5: P<0.05; Fig. 6). Double immunofluorescence was used to examine tight junctional protein expression in RBECs (Figs. S4-S6). Reduced ZO-1, occludin and claudin‑5 immunofluorescence was observed in the IL-1β group compared with that in the control group. Of note, ZO‑1, occludin and claudin‑5 fluorescence was enhanced with IL-1Ra pretreatment in RBECs.
Hypercapnia increases BBB permeability to EB in hypoxemic rats
An interaction effect was observed between hypoxia treatment and hypercapnia treatment (P<0.01; Fig. 7A). Simple effects analyses found increased extravasation of EB in the hypoxemia group (P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The extravasation of EB was the most prominent in the HH group compared with the hypoxemia group (P<0.01) and the hypercapnia group (P<0.01; Fig. 7B).
Discussion
The present study demonstrated that hypercapnia could increase BBB permeability through inducing IL-1β overexpression in the blood of hypoxemic rats. Additionally, it was demonstrated that hypercapnia decreased tight junctional protein expression in hypoxic cerebrovascular endothelial cells via the IL-1R1/p-IRAK-1 pathway. This was further verified by the increased expression of tight junctional proteins in hypoxic cerebrovascular endothelial cells when the effects of IL-1β were blocked by IL-1Ra.
The present rat model of hypercapnia/hypoxemia was established as described in our previous study (6). In the Sham group, the rats were exposed to air when the ventilator settings were fixed. The PaCO2 levels of the rats were maintained at 35-45 mmHg, and the PaO2 levels were maintained at 90-100 mmHg. The normal range of human PaCO2 and PaO2 is 35-45 and 80-100 mmHg, respectively, which are comparable between rats and humans. Treatment with 16% O2 maintained the PO2 levels of the arterial blood at ~60 mmHg, and 5% CO2 treatment maintained the PCO2 levels of the arterial blood at 60-70 mmHg, with the pH levels at 7.20-7.25. In vitro, 0.02% O2 treatment maintained the PO2 levels of whole-blood culture medium at ~60 mmHg. The PCO2 was ~80 mmHg, with the pH at 7.20-7.25 when treated with 10% CO2. The rats were treated with 5% CO2 and 10% CO2 was used to treat human whole-blood cultures. Although the CO2 concentrations were different in vivo and in vitro, the pH levels were consistent with the changes of permissive hypercapnia in ARDS (26-29).
The results of the present study demonstrated that hypercapnia enhanced the expression of IL-1β in hypoxic rats and whole-blood cultures. It has been reported that hypercapnia attenuated endotoxin‑induced inflammation through inhibiting nuclear factor (NF)-κB activation (21,30). The earlier research results appear to contradict those of the present study. However, the difference may be due to the underlying mechanism, whereby hypercapnia would exert its effects on IL-1β expression in the hypoxic blood. Endotoxin may induce IL-1β expression through the Toll-like receptor/NF-κB pathway (31-33). Hypoxia may also activate the NLRP3 inflammasome via inducing reactive oxygen species (ROS) overproduction, which can promote the secretion of IL-1β (34-37). In the present study, it was observed that hypercapnia alone may not be sufficient to increase the expression of IL-1β, but hypercapnia may exert its effects in the presence of hypoxia. It was suggested that hypercapnia can induce ROS overproduction via intensifying hypoxia, may activate the NLRP3 inflammasome and promote IL-1β release. In the central nervous system, it was previously demonstrated that hypercapnia-induced NLRP3 inflamma-some activation in hypoxia-activated microglia upregulated the expression of IL-1β (6). These findings are consistent with the effects of hypercapnia on IL-1β expression in the hypoxic blood in the present study.
To elucidate the mechanism through which hypercapnia can increase BBB permeability in hypoxemic rats through upregulating the expression of IL-1β, the expression levels of IL-1R1, p-IRAK-1 and tight junctional proteins (ZO-1, occludin and claudin-5) in cerebral vascular endothelial cells were examined. It was demonstrated that hypercapnia increased the expression of IL-1R1 and p-IRAK-1 and downregulated the expression of tight junctional proteins in hypoxic cerebral vascular endothelial cells. The expression of tight junctional proteins was markedly increased following treatment with IL-1Ra, an IL-1 receptor antagonist. IRAK-1 is critically involved in the regulation of intracellular signaling networks. IRAK-1 may perform distinct functions, including activation of NF-κB (38), which is closely associated with the disruption of the BBB (39,40). Taken together, these findings suggest that hypercapnia may aggravate BBB disruption in hypoxemic rats via inducing IL-1β overproduction. However, a limitation of the present study is that other cytokines (e.g., tumor necrosis factor-α) were not investigated, which should be addressed in future studies.
In summary, the present study demonstrated that hypercapnia serves as a stimulus triggering inflammation in the hypoxic blood. In this setting, hypercapnia may contribute to IL-1β overproduction in the hypoxic blood, and then down-regulate the expression of tight junctional proteins in the hypoxic cerebral vascular endothelial cells, thereby further disrupting BBB integrity in hypoxemic rats. The increased expression of tight junctional proteins in hypoxic cerebro-vascular endothelial cells when the effects of IL-1β are blocked by IL-1Ra further supports this hypothesis. Thus, the cascade of hypercapnia-induced IL-1β overproduction in hypoxic blood may be a potential target for ameliorating BBB disruption.
Supplementary Data
Acknowledgements
The authors would like to thank Zhengkang Ding and Zixi Yang for the technical support.
Abbreviations
- ARDS
acute respiratory distress syndrome;
- BBB
blood-brain barrier;
- IL-1β
interleukin-1β;
- IL-1R1
IL-1 receptor 1;
- IL-1Ra
IL-1 receptor antagonist;
- NF-κB
nuclear factor-κB;
- RBECs
rat brain capillary endothelial cells
Funding
This study was supported by the Natural Science Foundation of Guangdong Province (grant nos. 2016A030311043 and 2017A030313691), the Medical Scientific Research Foundation of Guangdong Province (grant nos. A2019135 and A2017284), the Scientific Research Project of Guangdong Provincial Bureau of Traditional Chinese Medicine (grant no. 20201045) and the Science and Technology Program of Guangzhou (grant no. 202002030338).
Availability of data and materials
The datasets generated and/or analyzed during the present study are available from the corresponding author on reasonable request.
Authors' contributions
HZ conceived the project and designed the experiments. HD carried out the assessment of BBB permeability and IL-1β evaluation by ELISA. XuL established the rat model of hypercapnia/hypoxemia. XiL carried out human whole-blood cultures and treatment. MW carried out cerebrovascular endothelial cell cultures and treatment. YH, LH and YL performed western blotting and immunofluorescence staining. HD and ML conducted the statistical analysis. HD and XuL wrote the manuscript. All the authors have read and approved the final version of the manuscript.
Ethics approval and consent to participate
The animal experimental protocols were approved by the Experimental Animal Care and Use Committee at Jinan University (approval no. 20171011001). The protocols involving human samples were approved by the Research Ethics Committee of Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China [approval no. GDREC2018424H(R1)] and all the participants provided written informed consent.
Patient consent for publication
Not applicable.
Competing interests
All the authors declare that they have no competing interests.
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Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Data Availability Statement
The datasets generated and/or analyzed during the present study are available from the corresponding author on reasonable request.