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. 2020 Jun 8;9:e56707. doi: 10.7554/eLife.56707

Figure 4. Correlation of fitness with structural attributes.

Figure 4.

Fitness scores are from DMS at 128 µg/mL AMP selection. (A) To the right, is the crystal structure of wtVIM-2 (PDB: 4bz3) colored by the average fitness of 20 amino acid mutations at each position. The inset to the left shows distributions of fitness scores for variants at positions with relative accessible surface area (ASA) <0.3 in wtVIM-2 or positions with ASA ≥0.3, with the number of variants in each distribution shown at the bottom and the results of a two-tailed Mann-Whitney U test between the two distributions at the top. (B) The correlation between accessible surface area and the average fitness of 20 amino acid mutations at each position. (C) The correlation between the changes in folding energy predicted by Rosetta and the DMS fitness scores for all variants.