Table 1.
Primers used for real-time PCR assays
| Gene (Accession No.) | Primer sequence (5’ to 3’) | Tm (°C) | Amplicon size (bp) |
|---|---|---|---|
| mGluR4 (NM_022666) |
F: CAGGTTATGCCACACACAGG R: GCAAAGACGGAAGAGAAACG |
60 | 178 |
| mGluR6 (NM_022920) |
F: GAACGTACAGAAGCGGAAGC R: GGTCGGGGTTTAGCTTTTTC |
60 | 249 |
| mGluR7 (NM_031040) |
F: CCAGGGCGTTATGACATCTT R: TATCCATCACAGGGCTCACA |
60 | 233 |
| mGluR8 (NM_022202) |
F: GGGCACTGGACAAATCAACT R: TGATCCAAAGGGCAAAGTTC |
60 | 215 |
| GAPDH (NM_017008) |
F: TGCCACTCAGAAGACTGTGG R: TTCAGCTCTGGGATGACCTT |
60 | 129 |
F, forward primer; R, reverse primer; Tm, primer melting temperature.