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. 2020 May 20;161(7):bqaa082. doi: 10.1210/endocr/bqaa082

Table 1.

Primers used for SYBR green-based quantitative real-time PCR analysis for gene expression

Target Forward Reverse
18s rRNA 5’-GGATGTAAAGG ATGGAAAATACA-3’ 5’-TCCAGGTCTTC ACGGAGCTTGTT-3’
KLF9 mRNA 5’-GAGCAGTCGCA GTGAGTTTA-3’ 5’-TCATCGGAGCA GATTTGGAAG-3’
ERRFI1 mRNA 5’- CTGGAGCAGT CGCAGTGAG-3’ 5’- GCCATTCATCGGAGCAGATTTG -3’
ERRFI1 pre-mRNA 5’- CTCTCGTTCA TTCCAGGGC-3’ 5’-AGCTGGACTT TTGAGATGGAC-3’
mErrfi1 mRNA 5’-CATCTCGAGTGAG GCAGTACACAGGAAG-3’ 5’-GCGAAGCTTGTG AAAGGCAGAATGAGTC-3’
ID2 mRNA 5’-ATCCCACTAT TGTCAGCCTGC-3’ 5’-TGAACACCGC TTATTCAGCCAC-3’
ID2 pre-mRNA 5’-CCTCTGCCCT TAGGTTACATT-3’ 5’-AAAGAAATC ATGAACTGC-3’
EGFR mRNA 5’-CAAGGAAGCCA AGCCAAATG-3’ 5’-CCGTGGTCAT GCTCCAATAA-3’
NR3C1 mRNA 5’-CCGTGGTCAT GCTCCAATAA-3’ 5’-CACCTTCCTGTC TCCTGTTTAC-3’