FIG. 1.

Overview schematic of bacterial enrichment and contact-free lysis driven by an AC magnetic field. Step 1. The syringe pump pushes sample through hexagonal microchannel. The external magnet retains bacteria bound to functionalized magnetic nanoparticles within the microchannel, while waste products are collected as the output. TEM image of S. aureus bound (∼0.5 μm) bound to magnetic nanoparticles (∼150 nm) (top right). Step 2. Overview schematic of contact-free cell lysis. External magnet is removed, microchip is placed in coil, and microchip is exposed to an AMF. Bacteria are thermally lysed, enabling downstream nucleic acid collection and analysis.