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. 2020 Jun 5;25(11):2636. doi: 10.3390/molecules25112636

Molecular Network-Guided Alkaloid Profiling of Aerial Parts of Papaver nudicaule L. Using LC-HRMS

Kwangho Song 1,†, Jae-Hyeon Oh 2,†, Min Young Lee 1, Seok-Geun Lee 1,3,*, In Jin Ha 1,4,*
PMCID: PMC7321159  PMID: 32517053

Abstract

Papaver nudicaule L. (Iceland poppy) is widely used for ornamental purposes. A previous study demonstrated the alleviation of lipopolysaccharide-induced inflammation mediated by P. nudicaule extract through nuclear factor-kappa B and signal transducer and activator of transcription 3 inactivation. As isoquinoline alkaloids are chemical markers and bioactive constituents of Papaver species, the present study investigated the alkaloid profile of aerial parts of five P. nudicaule cultivars with different flower colors and a P. rhoeas cropped for two years. A combination of liquid chromatography high-resolution mass spectrometry and molecular networking was used to cluster isoquinoline alkaloids in the species and highlight the possible metabolites. Aside from the 12 compounds, including rotundine, muramine, and allocryptopine, identified from Global Natural Products Social library and reported information, 46 structurally related metabolites were quantitatively investigated. Forty-two and 16 compounds were proposed for chemical profiles of P. nudicaule and P. rhoeas, respectively. Some species-specific metabolites showed similar fragmentation patterns. The alkaloid abundance of P. nudicaule differed depending on the flower color, and the possible chemical markers were proposed. These results show that molecular networking-guided dereplication allows investigation of unidentified metabolites. The derived chemical profile may facilitate evaluation of P. nudicaule quality for pharmacological applications.

Keywords: Papaver nudicaule, alkaloids, LC-MS/MS chemical profiling, GNPS molecular networking

1. Introduction

Plants in the Papaver L. genus from Papaveraceae (commonly known as poppy) family have been used for traditional medicinal practices and ornamental purposes for a long time. Papaver rhoeas L. (Corn poppy) and Papaver nudicaule L. (Iceland poppy) are widely used for ornamentation owing to their multicolored flowers. Nudicaulins, which are flavonoid-derived indole alkaloids, along with pelargonidin, kaempferol, and gossypetin glycosides, are responsible for the white, red, orange, and yellow colored petals of different P. nudicaule cultivars [1,2]. P. nudicaule belongs to the Papaveraceae family, and is an annual herbaceous species. This plant can produce various alkaloids, particularly isoquinoline alkaloids (IAs) [3]. IAs from Papaver species possess potent pharmacological properties, including narcotic analgesic, antimicrobial, muscle relaxant, cough suppressant, and anticancer effects [4,5]. However, studies on chemical constituents and biological activities of Papaver species have mainly been performed on P. somniferum, which produces morphine alkaloids [6].

To understand the metabolites of other Papaver species and their biological activities, the metabolite profiling of IAs in P. rhoeas was previously performed using liquid chromatography (LC) coupled with quadrupole time-of-flight (Q-TOF) mass spectrometry (MS) [7]. P. nudicaule extracts have anti-inflammatory activity and decrease the inflammatory response induced by lipopolysaccharide by inhibiting the nuclear factor-kappa B (NF-κB) and signal transducer and activator of transcription 3 (STAT3) signaling pathway in the previous report [8]. The study proposed that IAs, such as allocryptopine, from P. nudicaule could be the active constituents and a representative subset for this species. However, IAs of P. nudicaule have been rarely reported, except for flavonols; indole alkaloid skeleton of yellow petals [9,10,11] and some alkaloids of aerial parts, including 8, 14-dihydroflavinantine, pseudoprotopine, allocryptopine, dihydroamuronine, amuronine, amurensinine N-oxide A, and (−)-amurensinine N-oxide B [12,13].

Alkaloid profiling of P. nudicaule is crucial to investigate its pharmaceutical potential. However, a major challenge for MS-based chemical profiling of P. nudicaule is the identification of alkaloids. To the best of our knowledge, the compound information and MS/MS spectra of P. nudicaule have been rarely reported. Our main interest is to highlight the species-specific metabolites and propose a reliable alkaloid profile. Therefore, this study utilized the Global Natural Products Social (GNPS) molecular networking platform and significantly accelerated the MS/MS-based clustering of alkaloids, even for compounds with no spectral matches in any public database or in-house MS/MS library [14,15].

This study aimed to identify IAs and investigate their abundance between P. nudicaule and P. rhoeas, and further analyze the chemical markers in specimens from aerial parts of five cultivars of P. nudicaule based on liquid chromatography high-resolution mass spectrometry (LC-HRMS) and molecular network analysis.

2. Results

2.1. Molecular Network-Based Annotation of Species-Specific Metabolites

To organize the compound candidates for the alkaloid profiling of P. nudicaule and P. rhoeas, the LC-MS/MS data from 12 extracts was submitted to molecular networking through the GNPS web platform (http://gnps.ucsd.edu, ID: d38cc1e769524002ad2c2122f7375f08). The 12 extracts were prepared from the five cultivars of P. nudicaule with different flower colors (orange, purple, scarlet, white, and yellow), and a P. rhoeas sample was collected for two years (2017 and 2018). The extract from aerial parts of P. nudicaule with white bloom in 2017 is abbreviated as 17NW. The same format is followed for other extracts (i.e., orange, NO; purple, NP; scarlet, NS; and yellow, NY). The extract of aerial parts of P. rhoeas collected in 2018 is abbreviated as 18RA. The resulting molecular network comprised 2121 molecular features and 102 independent clusters with at least three features (Supplementary Figure S1). A total of six alkaloids were identified through MS/MS library matching of GNPS. The nodes belonged to two molecular clusters (Figure 1A). The two clusters comprising 58 molecular features were selected as species-specific alkaloids from the chemical profiles of P. nudicaule and P. rhoeas. This strategy was based on molecular networking using spectral similarity that allowed grouping of structurally related metabolites [16]. Although the molecular networking platform significantly increased structural annotations and clustering of Papaver species, the MS/MS library matching unfortunately failed to cover all metabolites. Indeed, 44 IAs were identified from P. rhoeas in the previous report [7], but the molecular network-guided profiling in this study missed some of these alkaloids. The unidentified alkaloids belong to other clusters that are not yet characterized are speculated.

Figure 1.

Figure 1

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(A) Alkaloid-targeted molecular network generated from the ethanolic extracts of P. nudicaule and P. rhoeas. Colors inside nodes represent species-specific metabolites: green, P. nudicaule; blue, P. rhoeas. (B) Nodes with red outer circles represent ions dereplicated by GNP libraries (*), authentic compounds (**), and literature (***). Identified ions are presented with their common names and chemical structures.

Within the 58 metabolites, six alkaloids were further annotated by manually comparing their MS/MS spectra with the in-house MS/MS library and previous literature [17,18]. The molecular network-guided approach reduced this laborious step by narrowing down the possible MS/MS features. Twelve alkaloids were identified (Figure 1B), and 46 metabolites were proposed to be structurally related alkaloids. Table 1 summarizes the chromatographic information (retention times), high-resolution MS/MS-based information (observed mass, formulas, MS/MS fragment ions), and quantitative information (abundant species, fold changes in peak areas) of each metabolite.

Table 1.

Molecular network-based alkaloids of P. nudicaule and P. rhoeas by liquid chromatography (LC)- quadrupole time-of-flight (QTOF)-mass spectrometry (MS)/MS.

Peak No. Rt (min) Observed Mass (Da) Calculated Mass (Da) Formula Adduct Form MS/MS Fragment Ions (m/z) Species Fold Change *
1 4.68 330.1326 330.1342 C19H24NO4 [M + H]+ 263.0700, 194.0804 *, 189.0688, 177.0769 *, 176.0703 * P. rhoeas −1.92
2 5.17 272.1274 272.1287 C16H18NO3 [M + H]+ 256.1035 *, 238.0969, 162.0625, 108.0529 *, 107.0501 * P. nudicaule 0.41
3 5.73 314.1745 314.1756 C19H24NO3 [M + H]+ 271.1322, 269.1156, 237.0897, 175.0758, 107.0499 * P. rhoeas −1.84
4 6.07 554.1616 554.1630 C38H22N2O3 [M + H]+ 352.1170 *, 334.1059, 190.0855 * P. rhoeas −1.98
5 6.16 286.1437 286.1443 C17H20NO3 [M + H]+ 270.1225 *, 238.0931, 210.0994, 108.0535 *, 107.0498 * P. nudicaule 1.10
6 6.27 300.1589 300.1600 C18H22NO3 [M + H]+ 270.1193 *, 238.0928, 176.0773, 108.0514 *, 107.0482 * P. nudicaule 1.07
7 6.31 358.1643 358.1655 C20H24NO5 [M + H]+ 277.0835, 191.0929, 190.0851 *, 151.0750 P. nudicaule 2.28
8 6.48 342.1335 342.1342 C19H20NO5 [M + H]+ 263.0708, 235.0753, 206.0803, 176.0704 *, 165.0543 P. rhoeas −1.92
9 6.48 368.1123 368.1134 C20H18NO6 [M + H]+ 350.1043, 332.0949, 261.0567, 188.0705 * P. rhoeas −1.67
10 6.64 342.1694 342.1705 C20H24NO4 [M + H]+ 297.1128 *, 282.0899, 265.0851 *, 237.0906, 191.0858 P. rhoeas −0.77
11 6.67 370.1282 370.1291 C20H20NO6 [M + H]+ 352.1191 *, 334.1087, 320.0927, 190.0865 *, 188.0710 * P. rhoeas −3.26
12 6.82 372.1424 372.1447 C20H22NO6 [M + H]+ 354.1340, 322.1152, 204.1051 *, 192.1013 P. rhoeas −0.81
13 6.91 618.1764 618.1791 C39H26N2O6 [M + H]+ 370.1305, 352.1171 *, 321.0745, 190.0835 P. rhoeas −2.60
14 6.94 535.2476 535.2444 C30H35N2O7 [M + H]+ 518.2158, 504.2009 *, 320.1053, 220.0970 *, 205.0732 P. nudicaule 3.35
15 7.11 344.1864 344.1862 C20H26NO4 [M + H]+ 298.1471, 267.1026, 191.0932, 190.0876 * P. nudicaule 0.56
16 7.12 358.1641 358.1655 C20H24NO5 [M + H]+ 340.1564, 278.0934, 194.0830, 176.0704 * P. nudicaule 0.33
17 7.27 370.1635 370.1655 C21H24NO5 [M + H]+ 338.1369, 238.0629, 192.1021 * P. nudicaule 2.07
18 7.38 372.1808 372.1811 C21H26NO5 [M + H]+ 354.1692, 291.1015, 222.1118, 204.1017 *, 190.0853 P. nudicaule 2.20
19 7.39 386.1581 386.1604 C21H24NO6 [M + H]+ 306.1193, 206.1168, 191., 190.0865 * P. rhoeas −1.67
20 7.78 356.1475 356.1498 C20H22NO5 [M + H]+ 340.1547 *, 325.1345, 267.0990, 192.1015 *, 177.0782 P. rhoeas −1.26
21 7.93 430.1854 430.1866 C23H28NO7 [M + H]+ 412.1746 *, 350.1138, 220.0961 *, 218.0798, 205.0839 P. nudicaule 2.25
22 8.00 402.1930 402.1917 C22H28NO6 [M + H]+ 384.1809 *, 335.1278, 206.1175 *, 193.0857, 179.0701 P. nudicaule 3.25
23 8.01 390.1907 390.1917 C21H28NO6 [M + H]+ 372.1808 *, 310.1199, 208.0969 *, 193.0724 P. nudicaule 2.85
24 8.05 356.1859 356.1862 C21H26NO4 [M + H]+ 206.1178 *, 190.0868, 162.0911 P. nudicaule 2.92
25 8.07 340.1547 340.1549 C20H22NO4 [M + H]+ 323.1095, 277.0838, 192.1016 *, 177.0790 P. rhoeas −1.67
26 8.30 352.1182 352.1185 C20H18NO5 [M + H]+ 334.1082, 320.0924, 190.0863 * P. rhoeas −3.99
27 8.97 356.1857 356.1862 C21H26NO4 [M + H]+ 325.1384, 249.1838, 192.1017 *, 177.0777 P. nudicaule 2.78
28 9.13 400.1745 400.1760 C22H26NO6 [M + H]+ 382.1670, 341.1352, 282.1280, 204.1006 *, 165.0901 * P. nudicaule 2.08
29 9.19 370.1650 370.1655 C21H24NO5 [M + H]+ 352.1534, 291.1014, 222.1122, 205.1094 *, 204.1018 * P. nudicaule 2.10
30 9.30 414.1901 414.1917 C23H28NO6 [M + H]+ 220.0969 *, 205.0740 P. nudicaule 3.84
31 9.38 388.1750 388.1760 C21H26NO6 [M + H]+ 370.1647 *, 352.1541 *, 336.1232, 322.1189, 308.1273 P. nudicaule 2.59
32 9.47 354.1338 354.1342 C20H20NO5 [M + H]+ 275.0710, 247.0758, 206.0812, 189.0779 *, 188.0708 * P. nudicaule 0.82
33 9.47 403.1974 403.1995 C22H29NO6 [M + H]+ 385.1845, 354.1717, 280.1043, 207.1207 *, 206.1171 * P. nudicaule 3.67
34 9.48 384.1797 384.1811 C22H26NO5 [M + H]+ 352.1535, 325.1429, 206.1183 *, 190.0870 P. nudicaule 3.83
35 9.48 402.1935 402.1917 C22H28NO6 [M + H]+ 384.1807 *, 353.1394, 325.1411, 206.1183 *, 190.0869 P. nudicaule 3.73
36 9.49 424.1749 424.1760 C24H26NO6 [M + H]+ 384.1911, 214.0871, 206.1176 * P. nudicaule 2.78
37 9.59 400.1747 400.1760 C22H26NO6 [M + H]+ 382.1643, 206.0815 *, 191.0586 P. nudicaule 3.57
38 9.60 422.1580 422.1604 C24H24NO6 [M + H]+ 351.1177, 206.0833 *, 191.0590 * P. nudicaule 2.14
39 10.05 386.1980 386.1968 C22H28NO5 [M + H]+ 368.1870, 306.1264 *, 222.1139, 204.1025 *, 190.0872 P. nudicaule 3.65
40 10.10 416.1691 416.1709 C22H26NO7 [M + H]+ 398.1606, 222.0767 *, 205.0733 P. nudicaule 2.41
41 10.48 370.1643 370.1655 C21H24NO5 [M + H]+ 290.0944 *, 206.0813, 188.0709 *, 181.0861 P. nudicaule 2.72
42 10.52 338.1385 338.1392 C20H20NO4 [M + H]+ 277.0861, 249.0912, 190.0859 *, 149.0596 P. rhoeas −1.67
43 10.80 384.1433 384.1447 C21H22NO6 [M + H]+ 352.1182 *, 334.1080, 320.0922, 190.0858 *, 188.0707 * P. rhoeas −4.37
44 11.04 370.2009 370.2018 C22H28NO4 [M + H]+ 206.1178 *, 190.0868 P. nudicaule 4.08
45 11.16 324.1227 324.1236 C19H18NO4 [M + H]+ 250.0942, 176.0707 *, 149.0596 * P. rhoeas −3.29
46 11.28 635.2425 635.2393 C37H35N2O8 [M + H]+ 499.2079, 398.1601 *, 380.1486, 220.0967 * P. nudicaule 2.05
47 11.70 500.1908 500.1921 C26H30NO9 [M + H]+ 456.2026, 397.1888, 220.0972 *, 205.0742 P. nudicaule 0.97
48 11.71 398.1595 398.1604 C22H24NO6 [M + H]+ 382.1282 *, 364.1176, 336.1231 *, 193.0859 P. nudicaule 3.51
49 11.78 386.1957 386.1968 C22H28NO5 [M + H]+ 222.1129 *, 161.0831 P. nudicaule 3.33
50 12.15 354.1695 354.1705 C21H24NO4 [M + H]+ 338.1361, 190.0864 *, 149.0593 P. nudicaule 3.11
51 15.51 402.1907 402.1917 C22H28NO6 [M + H]+ 384.1812 *, 322.1200, 220.0975 *, 205.0734 P. nudicaule 3.85
52 15.75 400.1748 400.1760 C22H26NO6 [M + H]+ 382.1649, 320.1051, 220.0965 *, 205.0736 P. nudicaule 3.47
53 16.00 663.2720 663.2706 C39H39N2O8 [M + H]+ 499.2075, 398.1603 *, 380.1482, 220.0972 * P. nudicaule 2.55
54 16.01 384.1793 384.1811 C22H26NO5 [M + H]+ 322.1197, 220.0970 *, 205.0729 P. nudicaule 2.77
55 16.15 444.2040 444.2022 C24H30NO7 [M + H]+ 384.1814 *, 322.1204, 220.0974 *, 205.0730 P. nudicaule 3.51
56 16.18 430.1852 430.1866 C23H28NO7 [M + H]+ 370.1661 *, 322.1185, 206.0810 *, 191.0576 P. nudicaule 2.35
57 16.30 499.2038 499.2022 C33H27N2O3 [M + H]+ 351.1147, 320.1046, 220.0969 *, 205.0733 P. nudicaule 2.94
58 16.36 430.2216 430.2230 C24H32NO6 [M + H]+ 384.1808 *, 353.1377, 206.1173 *, 192.1008 P. nudicaule 2.21
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* Relative fold changes between the two species were calculated from the ratio of the mean peak areas of P. nudicaule samples to that of P. rhoeas samples.

2.2. Characterized Alkaloids of P. nudicaule

Representative chromatograms for the selected IAs are shown in Figure 2. The major metabolites of P. nudicaule revealed some specific molecular ions upon MS/MS fragmentation. As shown in Figure 2A, compounds 30 and 52 generated fragment ions at m/z 220.10, whereas compounds 38 and 39 generated fragment ions at m/z 206.08 and m/z 204.10, respectively. The molecular ions at m/z 204.10, m/z 206.08, m/z 206.12 (data not shown), and m/z 220.10 could be diagnostic ions of P. nudicaule, as most major metabolites generated one of the fragment ions (Table 1). However, the major metabolites of P. rhoeas (compounds 11, 26, 42, and 43) were mainly fragmented to molecular ions with m/z 190.09 (Figure 2B).

Figure 2.

Figure 2

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Representative extracted ion chromatograms of P. nudicaule (A) and P. rhoeas (B) based on clustered alkaloids. Specific metabolites of each species were numbered according to their retention times, and the fragmentation patterns of major peaks are presented.

A relative quantification analysis was performed to explore the alkaloid abundance in P. nudicaule and P. rhoeas. Among the 58 compounds within two molecular clusters, 42 and 16 were abundant in P. nudicaule and P. rhoeas, respectively. As shown in Supplementary Table S1, the peak areas of each compound and the relative fold change between the two species were calculated. Considering the mean peak areas of P. nudicaule samples, species-specific alkaloids are summarized in Table 1 depending on fold change values. Furthermore, 12 identified alkaloids were quantitatively compared based on their peak areas. Figure 3 presents their abundance in 12 specimens harvested for two years (2017 and 2018) comprising five cultivars of P. nudicaule blooms and a P. rhoeas. Reticuline (1), armepavine (3), rhoeagenine (11), and rhoeadine (43) were more abundant in P. rhoeas. The levels of demethylcoclaurine (2), coclaurine (5), N-methylcoclaurine (6), rotundine (27), muramine (39), and allocryptopine (41) were much higher in P. nudicaule (Figure 3). Protopine (32) was detected in both species among protopine-type alkaloids.

Figure 3.

Figure 3

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The abundance of the identified alkaloids in P. nudicaule and P. rhoeas, as determined by liquid chromatography high-resolution mass spectrometry (LC-HRMS). Bars with white and black colors represent specimens of 2017 and 2018, respectively. Reticuline (1), demethylcoclaurine (2), armepavine (3), coclaurine (5), N-methylcoclaurine (6), rhoeagenine (11), rotundine (27), cryptopine (29), protopine (32), muramine (39), allocryptopine (41), and rhoeadine (43).

2.3. Alkaloid-Based Multivariate Analysis

A multivariate analysis was performed for 12 specimens based on molecular network-guided IAs. Figure 4 presents the K-means clustering for 58 identified alkaloids. Representative compounds are proposed. Compounds 23, 46, 52, 53, and 57 were significantly abundant in NW (Figure 4A), while compounds 14, 51, 54, and 55 were abundant in both NW and NS (Figure 4B). Compounds 21, 37, 38, 40, and 48 in NS (Figure 4C); 2, 5, and 47 in NP (Figure 4D); and 29, 31, and 58 in NO (Figure 4E) could be representative metabolites. None was represented for the specimen with yellow colored flower. Sixteen metabolites were significantly abundant in P. rhoeas as compared with those in P. nudicaule (Figure 4F), suggesting that alkaloid production in the two species depends on different biosynthesis pathways and that, even within the same species, the abundance of IAs is very different for each P. nudicaule cultivar depending on flower color. These results further the understanding of the biosynthesis of alkaloids in P. nudicaule, and the quantitative variation may explain the difference in biological activities within the same species. Protopine detected in both P. rhoeas and P. nudicaule (Figure 3) is also one of the IAs found in Sanguinaria canadensis L., another species in the family Papaveraceae. Protopine has various pharmacological and biological activities, including anti-inflammatory, anti-infectious, neuroprotective, and antithrombotic effects [19]. Further investigations should address the correlation between their chemical composition and biological activities.

Figure 4.

Figure 4

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Heatmaps of the molecular features illustrating K-means clustering for the 58 alkaloids of P. nudicaule and P. rhoeas derived from molecular network-guided analysis. Specimens from each analysis were hierarchically clustered. Representative metabolites were proposed for (A) NW, (B) NS and NW, (C) NS, (D) NP, (E) NO, and (F) RA.

3. Materials and Methods

3.1. Plant Material

The aerial parts of P. nudicaule L. and P. rhoeas L. were harvested by the Korea National Academy of Agricultural Science, Rural Development Administration. The five cultivars of P. nudicaule with different flower colors (orange, purple, scarlet, white, and yellow) and P. rhoeas samples collected for two years (2017 and 2018) were immediately frozen in liquid nitrogen and stored at −70°C in a deep freezer. For each specimen, experiments were performed in triplicate under the same conditions.

3.2. Reagents and Chemicals

The suppliers of the reference standard for IAs were previously described [5]. High-performance liquid chromatography (HPLC)-grade acetonitrile and methanol were purchased from Honeywell Burdick and Jackson (Morristown, NJ, USA). Formic acid and ammonium formate were procured from Sigma-Aldrich (St. Louis, MO, USA). Deionized water was obtained using a pure water purification system (Human Co., Seoul, Korea).

3.3. Sample Preparation

Aerial parts of lyophilized Papaver species were ground into a fine powder. Two grams of all samples were ultrasonicated in 5 mL of ethanol for 30 min. The supernatants were filtered through 0.2 μm polytetrafluoroethylene syringe filters and dried using a SAVANT SPD2010 SpeedVacTM concentrator (Thermo Scientific, Asheville, NC, USA). Ethanolic extracts were dissolved in HPLC-grade methanol to 2.0 mg/mL and either directly analyzed or stored at −70°C until analysis.

3.4. LC and MS Analysis

The LC-MS system comprised a Vanquish UHPLC system (Thermo Fisher Scientific, Sunnyvale, CA, USA) with an Acquity UPLC HSS T3 column (2.1 mm × 100 mm, 1.7 μm; Waters) and a Triple TOF 5600+ mass spectrometer system (Sciex, Foster City, CA, USA). The ultrahigh performance liquid chromatography (UHPLC) system used 0.05% formic acid and 2.5 mM ammonium formate in water as eluent A and acetonitrile as eluent B. The optimized elution program was as follows: 0–2.5 min (1% B), 2.5–3.0 min (1–10% B), 3.0–6.0 min (10–19% B), 6.0–9.0 min (19–22% B), 9.0–14.0 min (22–25% B), 14.0–17.0 min (25–70% B), 17.0–19.0 min (70–100% B), 19.0–22.0 min (100% B), and equilibration with 1% B for 3 min at a flow rate of 0.4 mL/min. The column was maintained at 40 °C, and the auto-sampler was held at 4 °C. The injection volume of each sample solution was 1 μL. The MS/MS data were acquired by an information-dependent acquisition scan at positive-ion mode, and the parameters were as follows: mass range 50–1500 m/z, ion spray voltage, 4.5 kV; source temperature, 450 °C; declustering potential, 50 V; nitrogen as nebulizer gas, 50 L/min; heater gas, 50 L/min; curtain gas, 25 L/min; and collision energy, 10 eV.

3.5. LC-MS/MS Data Processing

The acquired AB Sciex dataset (.wiff) was directly imported into MZmine 2.53 [20]. Each detection of ms1 and ms2 levels was filtered with ions showing a minimum height 1.0 × 103 and 5, respectively, and the extracted ion chromatograms (XICs) were built with ions showing a minimum time span of 0.02 min, a minimum height of 1.0 × 103, and an m/z tolerance of 0.002 Da (or 10.0 ppm). The chromatograms were deconvoluted by a baseline cut-off algorithm using the following parameters: minimum peak height 1.0 × 103, peak duration range 0.02–0.25 min, and baseline level 1.0 × 10. The deconvoluted peaks were deisotoped using an isotopic peaks grouper algorithm with an m/z tolerance of 0.002 Da (or 10.0 ppm) and a retention time tolerance of 0.1 min, and aligned together into a peak list using a join aligner module and following parameters: m/z tolerance at 0.005 Da (or 20.0 ppm), weight for m/z of 70, absolute retention time tolerance of 0.1 min, and weight for m/z of 30. The chromatogram was gap-filled by a peak finder module with an intensity tolerance of 10.0%, m/z tolerance of 0.002 Da (or 10.0 ppm), and absolute retention time tolerance of 0.2 min. The processed peak list was eventually exported to an mgf file using the GNPS-FBMN module for GNPS molecular networking [21].

3.6. Identification of Species-Specific Metabolites Using Molecular Network Processing

The MZmine-processed LC-MS/MS peak lists were window-filtered by choosing only the top six peaks in the ± 50 Da window throughout the spectrum. A network was created where edges were filtered to have a cosine score > 0.70 and more than four matched peaks. Further edges between two nodes were retained in the network only if each node appeared in every other’s respective top-10 most similar nodes. The spectra in the network were then searched against the spectral library of GNPS. The library spectra were filtered in the same manner as the input data. The molecular network was visualized using Cytoscape 3.7.2 [22].

3.7. Multivariate Analysis

Identified alkaloids and peak areas were scaled by unit variance based on the year of specimen collection. The scaled data were subjected to K-means clustering and hierarchical tree construction using Multi-experiment Viewer (MeV) version 4.9.0 [23]. The K-means clustering was performed using Pearson’s correlation with 12 cluster numbers and optimized order of molecular features, and the hierarchical trees were constructed using complete linkage clustering with optimized order of sample leaf.

4. Conclusions

In the present study, a combination of LC-HRMS and molecular networking was applied to cluster structurally related compounds in natural products and highlight the possible metabolites important for the chemical profiling of the species. As a case study, IAs from P. nudicaule and P. rhoeas were investigated. The chemical annotation was significantly increased using the molecular networking platform. A total of 58 metabolites were selected and quantitatively analyzed. Forty-two and 16 compounds were proposed as chemical profiles of P. nudicaule and P. rhoeas, respectively. The alkaloid abundance of P. nudicaule was different depending on the color of flowers. These results suggest that the LC-HRMS and molecular networking-guided dereplication method can be a powerful tool for the chemical investigation of unidentified metabolites and chemotaxonomic approaches in the phytomedicine field. Together, these results may contribute to the understanding of the biosynthesis of alkaloids in P. nudicaule and the evaluation of the quality of this plant for further pharmaceutical applications.

Acknowledgments

We would like to thank Editage (www.editage.co.kr) for English language editing.

Supplementary Materials

The following are available online, Figure S1: Molecular network from ethanolic extracts of Papaver nudicaule and Papaver rhoeas; Table S1: LC-MS/MS information for clustered metabolites.

Click here for additional data file. (389.9KB, zip)

Author Contributions

Conceptualization, K.S., J.-H.O., and I.J.H.; Methodology, K.S.; Software, K.S., M.Y.L.; Formal analysis, K.S., M.Y.L.; Investigation, K.S., J.-H.O., Resources, J.-H.O., S.-G.L., and I.J.H.; Data curation, K.S.; Writing–original draft preparation, K.S., J.-H.O.; Writing–review and editing, S.-G.L., I.J.H.; Supervision, S.-G.L., I.J.H.; Project administration, S.-G.L., I.J.H.; Funding acquisition, J.-H.O., S.-G.L., and I.J.H. All authors have read and agreed to the published version of the manuscript.

Funding

This research was supported by a grant from the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2018R1D1A1B07047610) and was carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01045502)" Rural Development Administration, Republic of Korea.

Conflicts of Interest

The authors have declared no conflict of interest.

Footnotes

Sample Availability: Samples of the examined materials are available from the authors.

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