Table 1.
Confocal fluctuation analysis of cytosolic LRRK2-GFP constructs.
| Protein a | Concentration (nM) | Average Diffusion (µm2/s) | Average ε (cpsm) | Normalized ε c |
|---|---|---|---|---|
| mGFP | 30 ± 10 | 9.0 ± 2.0 | 35,000 ± 1,000 | 1.0 ± 0.1 |
| mLRRK2; dLRRK2; tLRRK2 | N.A. | 4.9 | 35,000 | 1.0 |
| 4.0 | 70,000 | 2.0 | ||
| 2.9 | 140,000 | 4.0 | ||
| WT LRRK2-GFP |
205 ± 85 | 2.4 ± 1.1 | 43,000 ± 9,000 | 1.2 ± 0.3 |
| G2019S LRRK2-GFP |
175 ± 100 | 2.0 ± 0.9 | 72,000 ± 15,000 b | 2.1 ± 0.5 |
a—For each condition (mGFP, WT LRRK2-GFP, G2019S LRRK2-GFP) with calculated concentrations of transfected proteins (n ≥ 20 cells), no significant trend was found for concentration and thus each concentration was included in the final calculations. Monomeric (mLRRK2), dimeric (dLRRK2), and tetrameric (tLRRK2) are calculated theoretical values for the purpose of comparing cytosolic properties of the experimental conditions. Therefore, there are no reportable concentrations for these species. b—p < 0.05 from a two-sample t-test when control sample, mGFP, was compared to the experimental conditions. c—Normalized brightness values were generated by dividing the brightness of the sample over the monomer control. Error is reported as ± standard deviation.