Correction: DAPLE protein inhibits nucleotide exchange on Gαs and Gαq via the same motif that activates Gα_i.

VOLUME 295 (2020) PAGES 2270-2284

MgCl₂ was omitted as a reagent in two buffers described under “In vitro protein-binding assays with GST-fused proteins” section (under “Experimental procedures”). “Beads were washed twice with PBS and resuspended in 300 µl of binding buffer (50 mm Tris-HCl (pH 7.4), 100 mm NaCl, 0.4% (vol:vol) NP-40, 5 mm EDTA, 2 mm DTT)” should read as “Beads were washed twice with PBS and resuspended in 300 µl of binding buffer (50 mm Tris-HCl (pH 7.4), 100 mm NaCl, 10 mm MgCl₂, 0.4% (vol:vol) NP-40, 5 mm EDTA, 2 mm DTT).” “Beads were washed four times with 1 ml of wash buffer (4.3 mm Na₂HPO₄, 1.4 mm KH₂PO₄, pH 7.4, 137 mm NaCl, 2.7 mm KCl, 0.1% (vol:vol) Tween-20, 5 mm EDTA, 1 mm DTT)” should read as “Beads were washed four times with 1 ml of wash buffer (4.3 mm Na₂HPO₄, 1.4 mm KH₂PO₄, pH 7.4, 137 mm NaCl, 2.7 mm KCl, 0.1% (vol:vol) Tween-20, 10 mm MgCl₂, 5 mm EDTA, 1 mm DTT).”