Abstract
Previously, using simultaneous resting-state functional magnetic resonance imaging (fMRI) and photometry-based neuronal calcium recordings in the anesthetized rat, we identified blood oxygenation level-dependent (BOLD) responses directly related to slow calcium waves, revealing a cortex-wide and spatially organized correlate of locally recorded neuronal activity (Schwalm et al., 2017). Here, using the same techniques, we investigate two distinct cortical activity states: persistent activity, in which compartmentalized network dynamics were observed; and slow wave activity, dominated by a cortex-wide BOLD component, suggesting a strong functional coupling of inter-cortical activity. During slow wave activity, we find a correlation between the occurring slow wave events and the strength of functional connectivity between different cortical areas. These findings suggest that down-up transitions of neuronal excitability can drive cortex-wide functional connectivity. This study provides further evidence that changes in functional connectivity are dependent on the brain’s current state, directly linked to the generation of slow waves.
Research organism: Rat
Introduction
Spatio-temporal dynamics of cortical excitability are constantly impacted by fluctuations of internally generated activity, even in the absence of external inputs. The resting-state of the cortical functional architecture is characterized by canonical ‘default-mode networks’ (Fox and Raichle, 2007; Mitra and Raichle, 2016; Raichle, 2015) which have been identified in humans (Raichle, 2011), primates (Chauvette et al., 2011) and rodents (Gozzi and Schwarz, 2016; Jonckers et al., 2011). However, the spatio-temporal features of these networks are not static (Deco et al., 2013). Alternating states of excitability, manifesting as different activity states of the brain, can dynamically occur, even in different regions at the same time, as seen in the awake condition (Guo et al., 2004). Different states of brain activation can influence responses upon incoming sensory afferents (Pachitariu et al., 2015; Schwalm et al., 2017) and are accompanied by alterations in the spatiotemporal pattern of neuronal activity in many brain areas (Pais-Roldán et al., 2020). These alterations can be observed in local electrophysiological (Constantinople and Bruno, 2011; Deco et al., 2013; Gozzi and Schwarz, 2016) and optical (Jonckers et al., 2011; Matsui et al., 2016) recordings, as well as in large-scale readouts of brain activity, such as BOLD fMRI (Kalthoff et al., 2013; Lewis et al., 2009; Staresina et al., 2013). The ubiquity of fluctuating activity states which influence ongoing brain dynamics calls for a state-dependent assessment of resting state functional connectivity (Tagliazucchi and Laufs, 2014).
Recently, using local and brain-wide readouts, we have identified two activity states which were shown to occur under different types of anesthesia used for small animal functional magnetic resonance imaging (fMRI) studies (Schwalm et al., 2017). In a state of persistent activity, which can occur during periods of light anesthesia, sedation or during awake periods (Constantinople and Bruno, 2011), neurons are rather depolarized, sparsely active, leading to temporally dynamic, modality specific, network configurations (Barth and Poulet, 2012; D'Souza et al., 2014). In contrast to persistent activity, stands the bimodal activity pattern of slow oscillations, or slow waves which have been extensively described under various conditions (Chauvette et al., 2011; Ma and Zhang, 2018; Petersen et al., 2003; Sanchez-Vives et al., 2017; Sanchez-Vives and McCormick, 2000; Schwalm et al., 2017; Seamari et al., 2007; Stroh et al., 2013; Zucca et al., 2017), but only most recently in the framework of BOLD fMRI (Chang et al., 2016; Liu et al., 2011; Mitra et al., 2015; Schwalm et al., 2017). Their corresponding low-frequency component ranges at 0.2–1 Hz, reflecting alternating activity patterns: active (‘up’) periods in which cells are depolarized and fire action potentials in temporally restricted periods, and silence (‘down’) periods with rather hyperpolarized membrane potentials and an almost complete absence of neuronal activity (Destexhe et al., 2007; Steriade et al., 2001). Slow waves can occur rather locally (Nir et al., 2011) or can spread over the entire cortex (Ma and Zhang, 2018; Schwalm et al., 2017), eventually allowing activity to propagate (Sanchez-Vives et al., 2017). In our previous work, we found that independent component analysis (ICA) for data obtained during persistent activity revealed canonical resting-state associated networks, such as the default mode (Lu et al., 2012; Schwalm et al., 2017) or different sensory networks (Liang et al., 2013; Pawela et al., 2008). Defining different states of brain activity by their local and global features of network dynamics by no means entails that their behavioral correlates, as for example in an anesthetized condition versus natural sleep, would be identical. However, defining features of slow wave activity, such as its bimodality, are resembled in a plethora of different conditions (Bullmore and Sporns, 2009; Ma and Zhang, 2018; Mitra et al., 2018; Stroh et al., 2013), likely because the fundamental difference between fast and slow timescale cortical dynamics is regulated by distinct mechanisms at the single neuron level (Okun et al., 2019).
Functional connectivity analysis for resting-state fMRI is used to identify and characterize neural networks in health and disease (Buckner et al., 2005; Woodward et al., 2011), during learning (Lewis et al., 2009) or memory consolidation (Staresina et al., 2013). The possibility to combine resting-state fMRI with invasive recordings of brain activity constitutes a pivotal branch of translational research (2018). Despite evidence of subtle changes in anesthesia level being able to dramatically change brain connectivity (Grandjean et al., 2014; Hutchison et al., 2014; Zhang et al., 2019), little has been investigated on how the brain’s current state impacts functional connectivity derived from blood oxygenation level-dependent (BOLD) activity. While variations in functional connectivity have been related to cognitive state in humans (Finn et al., 2017), the effect of neurophysiologically defined states on functional connectivity in the cortex remains unknown. Directly relating functional connectivity to defined brain states may have explanatory value for a broad range of functional brain connectivity studies. We propose that connectivity measures in different conditions such as different anesthetics (Bukhari et al., 2017; Kalthoff et al., 2013; Liu et al., 2013; Nasrallah et al., 2014; Paasonen et al., 2018; Shin et al., 2016) can be classified based on the respective activity state of the brain. Based on these classifications, meaningful comparisons can be drawn both between functional connectivity signature in rodents as well as in humans.
Here, we find distinct BOLD functional connectivity patterns for a state of persistent activity and for a state dominated by the occurrence of slow waves. We identified BOLD correlates revealing compartmentalized network activity during the persistent activity state and a cortex-wide component during slow wave activity. Furthermore, we show a cortex-wide functional coupling of brain activity during slow wave activity which has its origin in transitions from population down to population up states which occur during ongoing slow wave activity. Our data reveals the imminent consequences of each slow wave event for the configuration of dynamic functional networks during this type of brain-wide activity.
Results
We conducted ICA of rat resting-state fMRI signals during isoflurane-induced slow wave activity and in a medetomidine-induced persistent activity. For slow wave activity data, we found a characteristic cortex-wide component as characterized previously (Schwalm et al., 2017). Conversely, during persistent activity, this cortex-wide component was absent and canonical independent components of well-compartmentalized default mode networks were prevailing. While we found a cortex-wide component in all animals during isoflurane-induced slow wave activity (Figure 1A), the identified components for persistent activity resembled those described by others for awake (Becerra et al., 2011; Paasonen et al., 2018) or sedated rodents (Bukhari et al., 2018; Schwalm et al., 2017). We identified default mode network activation in 14 of 15 animals (Figure 1B), a bilateral auditory component in 11 of 15 medetomidine-sedated animals (Figure 1C) and a bilateral component of the insula in 9 of 15 animals (Figure 1D). All the selected components of each animal can be observed in Figure 1—figure supplement 1. Similarly, as it has been reported in previous work (Hsu et al., 2016), we identified four default mode network regions: left and right posterior parietal cortex, anterior and posterior insular cortex and orbital/prelimbic cortex. To corroborate the difference in the network organization between both brain states, we then manually defined nine anatomical regions of interests (ROIs) based on the z-scores maps of the persistent activity components. We run a functional connectivity analysis of each ROI for each individual and plotted the resulting matrix. When both matrices are compared, it can be observed that there is a wide distribution of significant connectivity among the different networks (delineated by the black squares) during slow wave activity (Figure 1E) compared with the high specificity showed during persistent activity (Figure 1E). These results indicate that there are marked differences in the network configurations between both types of activity.
Persistent and slow wave activity display different functional connectivity networks
To assess whether the cortical connectivity patterns previously described belong to those particular networks or are illustrating a general cortical phenomenon, we obtained the functional connectivity signature of the cortical networks during slow wave and persistent activity. We parcellated the brain based on anatomical landmarks using the Valdes-Hernandez et al template for rats (Valdés-Hernández et al., 2011) and correlated the BOLD signal of 96 cortical structures using the average signal from the white matter structures, ventricles and the animal’s breathing rate as covariables in both conditions (Figure 2A). The name and order of cortical areas can be found in Figure 2—figure supplement 1. We plotted the cumulative distribution function (CDF) for the r-values of each matrix (n = 15) to establish whether a difference in the number and strength of significant correlations can be found. The cutoff was calculated for each matrix using FDR (mean 0.379 ± 0.041 s.e.m.). The CDF values differed significantly between both activity states (paired t-test, t(14) = 6.01 p<0.001) at the mean of the cutoff (Figure 2B). The number of correlated pairs for each individual at its particular cutoff was significant (paired t-test, t(14) = 6.502, p<0.001) (Figure 2C). These results suggest that slow wave and persistent activity are related to different functional connectivity patterns. To illustrate this point, we plotted connectivity patterns between regions in both brain states (Figure 2C and D), from this diagram it is possible to infer that during persistent activity, functional connectivity is more compartmentalized than during slow wave activity.
Figure 2. Increased functional connectivity during isoflurane-induced slow wave activity compared to persistent activity related to medetomidine sedation.
(A) Average of the correlation matrices (n = 8) of the mean BOLD signal in 96 cortical regions for slow wave activity (magenta) and persistent activity (green). (B) Cumulative distribution curve for the r-values of the correlations. The vertical gray line lies at the mean of the FDR values used as a cutoff to identify significant correlations, ***p<0.001, paired t-test. (C) Box plot of the amount of significant correlations for each individual animal in both activity states ***p<0.001, paired t-test, vertical dashed line represents the data distribution, central horizontal bar the median and the two extremes horizontal bars point the interquartile range. (D) Diagram of the significant cortical connections during slow wave activity. Each line represents significant connections between cortical nodes (96 in total). Circles signaling the nodes are plotted in the center of the ROI. (E) Similar than D but for the persistent activity networks.
Figure 2—figure supplement 1. Cortical areas used for connectivity analysis.
Population activity during the presence of slow waves is characterized by long periods of quiescence followed by short lapses of neural activity corresponding to a propagating wave (Stroh et al., 2013). Frequency and power distributions of the BOLD signal and their relationships to functional connectivity have been studied before with respect to specific anesthetic conditions (Grandjean et al., 2014; Paasonen et al., 2018). fALFF analysis allows to determine the fractional power of the BOLD signal for a particular region of interest (ROI) (Zou et al., 2008). To rule out that the connectivity values observed during slow wave activity are due to the spurious consequence of an increase in power, we performed a fALFF analysis to investigate the differences in the BOLD power (Figure 3A), showing that during slow wave activity there is a significant shift to lower power values in the distribution of the fALFF curves which were created with the 96 cortical ROIs of each animal (paired t-test, t(14) = 3.11, p=0.008). To test the dependence of the strength in connectivity from the distance of cortical areas, we correlated the Euclidean distance between pairs with the significant r-scores of the correlation of those pairs (Figure 3B). The closer to 90° angle the slope of those correlations gets, the more dependent of the Euclidean distance their correlation becomes. We found, that this is indeed the case for slow wave activity recordings (Figure 3C), whose slopes are significantly lower than those of data recorded during persistent activity (paired t-test, t(14) = 5.43, p<0.001). These results corroborate that the BOLD signal during slow wave activity is governed by different dynamics than the BOLD signal during persistent activity. Furthermore, during slow wave activity, BOLD-driven connectivity seems to be less compartmentalized and shows a stronger dependency on the distance between areas than it is the case for persistent activity.
Figure 3. Network dynamics differ for slow wave and persistent activity.
(A) Plot of the fALFF analysis. The fALFF value was obtained for each region of interest and then a normal distribution was fit with the values of the 96 cortical ROIs for each individual during slow wave (n = 15, magenta) and persistent activity (n = 15, green), ***p<=0.001. The average values of the distribution for each condition is represented by the thick line. (B) Correlation between the r-scores of each pair of cortical ROIs and their Euclidean distance, **p<=0.01. Each point corresponds to an r-score of a particular pair of ROIs. Linear correlation between ROIs and distance is plotted for persistent (n = 15, green) and slow wave activity (n = 15, magenta). The means of the correlations for each condition and the standard error of the mean are plotted in thicker lines. (C) Box plot of the slopes for each individual plotted in B. ***p<=0.001, paired t-test (n = 15). vertical dashed line represents the data distribution, central horizontal bar the median and the two extremes horizontal bars point the interquartile range.
Cortical small world network architecture characterizes persistent activity and disappears during slow wave activity evidenced by graph theory analysis
To assess the organizational nature of the cortical networks identified by our previous analyses, we applied graph theory methods (Rubinov and Sporns, 2010). We first computed the modularity degree of the cortical networks identified during persistent and slow wave activity respectively. Modularity is a quality index for a partition of a network into non-overlapping communities (Schwarz et al., 2008). Therefore, in a brain with more compartmentalized networks this measure is higher than in one that shows a less selective network configuration. When we compared modularity values from networks identified for slow wave activity to those of networks identified for persistent activity, we indeed observed significantly higher values for persistent activity (paired t-test, t(14) = 4.01, p=0.001; Figure 4A), resembling results previously reported by others (D'Souza et al., 2014). Global and local efficiency are two important characteristics to differentiate small world from random networks. Small world networks exhibit high values for both measurements, whilst randomized networks show high values in global but low values for local efficiency (Latora and Marchiori, 2001). When we compared these measures, we did not find significant differences in the global efficiency scores (Figure 4B; paired t-test, t(14) = 0.389, p=0.703), but we found significantly lower scores for slow wave activity than for persistent activity in the local efficiency measurement (Figure 4C; paired t-test, t(14) = 5.869, p<0.001). It is well documented that both, small world and random networks show a short characteristic path length, whilst only small world networks show a high clustering coefficient (Watts and Strogatz, 1998). In our analyses, we found a significantly lower clustering coefficient for networks dominated by slow wave activity (Figure 4D; paired t-test, t(14) = 3.789, p=0.002), but no significant differences between networks in slow wave and persistent activity for characteristic path length (Figure 4E; paired t-test, t(14) = 0.636, p=0.535). This suggests that during slow wave activity the cortex rather behaves like a random network, whilst during persistent activity it exhibits small world network features (Figure 4F). In summary, graph theory analysis indicated a different functional connectivity pattern for persistent and slow wave activity. For persistent activity canonical well compartmentalized, small world networks seem to play a dominating role, while during slow wave activity a highly interconnected randomized network is prevalent.
Figure 4. Graph theory shows a random network signature during slow wave activity.
Legend: *=p < 0.05; **=p < 0.01; ***=p < 0.001 (paired t-test n = 15); slow wave activity (magenta), persistent activity (green). vertical dashed line represents the data distribution, central horizontal bar the median and the two extremes horizontal bars point the interquartile range. (A) Modularity values. (B) Global efficiency values. (C) Local efficiency values. (D) Clustering coefficients. (E) Characteristic path lengths. (F) Diagram representing a characteristic configuration of small world (green) and randomized networks (magenta).
Different activity states of the brain are reflected by changes in functional connectivity
Functional connectivity patterns in the rodent brain can change dramatically depending on the anesthetics used (Bukhari et al., 2017; Matsui et al., 2016; Wu et al., 2017). In order to demonstrate our effects to be independent of the anesthetic agent, we compared the medetomine-induced sedation persistent activity to a condition of low isoflurane anesthesia (0.5% - 0.7%) that prevents animals of entering slow wave activity (n = 6 animals), hypothesizing that both protocols lead to a similar functional state, that is persistent activity. In the fiber photometry recordings (Figure 5A), it becomes apparent that the population dynamics recorded under this low isoflurane anesthesia regime resemble the ones obtained under medetomidine, rather than showing similarity to the ones recorded under higher isoflurane concentrations (Figure 5B). To quantify whether this observation is meaningful for the BOLD signal, we computed the functional connectivity analysis in the same way as described for the previous experiments and obtained the connectivity matrices (Figure 5C). We computed matrix similarity analysis for each of the six matrices against the mean matrix for slow wave and persistent activity to determine if the connectivity patterns obtained under the low isoflurane concentration rather resemble the ones previously measured under medetomidine, (persistent activity) or the ones measured under higher isoflurane concentrations (slow wave activity). Similarity values can be compared extracting the eigen values of the matrix and then using Frobenius norm in order to calculate their similarity. The lower the obtained value, the greater is the similarity between two matrices. In this case, the resulting values related to persistent activity induced by low isoflurane, were significantly lower when compared with the ones for persistent activity induced by medetomidine sedation than when compared to the ones related to slow wave activity under high isoflurane concentration (Figure 5D; paired t-test, t(5) = 5.19, p=0.003). We used the same matrix similarity computation in a dynamic connectivity analysis. Therefore, we compared the initial 5 min of recordings with a moving average of 5 min in steps of 1 min. The results show that in the case of persistent activity induced by medetomidine and by low isoflurane concentrations, network configurations were significantly more stable than in the case of slow wave activity induced by high isoflurane concentrations (Figure 5E). This indicates that the network dynamics during slow wave activity might be related to a constant reorganization of connectivity patterns, probably due to the frequent changes in the rate of population down-up transitions.
Figure 5. Different activity states of the brain result in characteristic functional connectivity matrices.
(A) Calcium fiber photometry schematic. OGB-1 was bolus-injected and an optic fiber with a diameter of 200 µm was implanted at a cortical depth of about 300 μm. Blue light was used for excitation of OGB-1. Emitted fluorescence, comprising the changes in cytosolic calcium concentration of the local neural population was collected by the same fiber, and recorded by the fiber optometer. (B) Characteristic signal traces of photometry recordings during slow wave activity induced by high isoflurane (SW-I, magenta), and persistent activity induced either by medetomidine (P-M, green) or by lower concentrations of isoflurane (blue, P–I). (C) Average of the correlation matrices (n = 6) of the mean BOLD signal in 96 cortical regions under low isoflurane concentration. (D) Matrix similarity analysis of the low isoflurane (P-I n = 6) persistent activity experiments compared with the average matrix generated under high isoflurane related slow wave activity (SW-activity, magenta) and the medetomidine-induced persistent activity (P-M, green), **p<0.01, (n = 6, paired t-test). (E) Matrix similarity observed under the three conditions under a dynamic connectivity analysis. Lines represent the average of individual variability for slow wave activity (SW-I, n = 15, magenta), persistent activity induced with medetomidine (P-M, n = 15, green) and persistent activity induced with isoflurane (P-I, n = 6 blue), the semitransparent stripe correspond to the standard error of the mean for each condition. The matrices were generated for a 5-min period with steps of 1 min and were compared with the average matrix generated in the first 5 min for each individual animal, **p<0.01 (paired t-test n = 15 in SW-I and P-M conditions).
Population down-up transitions drive functional connectivity during slow wave activity
Slow wave activity has been proposed to constitute the default activity pattern of the cortex, which is present during deep slow wave sleep or when connections with the thalamus are physically removed (Sanchez-Vives et al., 2017). Local occurrences of slow wave activity have also been shown to take place in cortical circuits during awake periods (Vyazovskiy et al., 2011). This bimodal pattern – periods of neural quiescence interrupted by short bursts of activity - generates waves of activity that can propagate across the cortex (Busche et al., 2015; Stroh et al., 2013). We recently showed that these waves generate a cortex-wide increase in BOLD activity (Schwalm et al., 2017) which may also be responsible for a cortex-wide increase of functional connectivity during slow wave activity. To test this, we analyzed the previously obtained functional connectivity results during slow wave activity while using the cortex-wide component as a covariable, since this component represents a direct correlate of local slow waves (Schwalm et al., 2017). If the high connectivity signature found during slow wave activity is related to population down-up transitions, the cortex-wide component as a covariable should remove any correlation that occurs due to such down-up transitions observable in the population signal. Following this rationale, we expected that once the down-up transition covariable is removed from the connectivity analysis, correlation values should significantly drop (Figure 6Ai). Therefore, the cumulative distribution function of the r-values obtained using the cortex-wide component as a covariable (CWCcov) should be shifted to the left compared with those obtained using the original cortex inverted signal of the cortex-wide component (CWCinv). The CDF data (same analysis as in Figure 2B) showed significantly higher values for the CWCcov condition at the mean of the cutoff (Figure 6Aii) (paired t-test¸ t(14) = 4.96, p<0.001). Furthermore, the amount of significantly correlated pairs for each individual at its particular cutoff was also significantly less in the CWCcov condition. (paired t-test, t(14) = 5.435, p<0.001).This suggests a relationship between slow wave activity-related down-up transitions in the population signal and the strength of cortical functional connectivity. Additionally, we co-registered calcium photometry (Adelsberger et al., 2014; Grienberger et al., 2012) and fMRI BOLD signals during slow wave activity in five animals (Figure 6Bi). We quantified the number of population down-up transitions in a period of 3 min in the fluorescence signal emitted by a neural population in visual cortex (V1) (Figure 6Bii, upper panel) and correlated it with the number of significant pairs from the functional connectivity analysis of the BOLD signal for the same time period (Figure 6Bi, lower panel). We quantified these values for 30 min-long recordings using samples of 3 min and a gap of 1 min in between them. The correlation between the amount of down-up transitions in the fluorescence signal and the BOLD pairs was significant for five of the six animals (p<0.001) (Figure 6Bii). In order to overcome a potential source of a spurious correlation, we run a permutation test on each resulting correlation. The permutation test allows to establish the baseline correlation between points and to calculate the distance of the obtained results from that correlation. In the five animals, the permutation test shows that the correlation r-scores are in the 5% of significant values (Figure 6Bii, normal distribution plots). Finally, we calculated the statistical value for the whole dataset using the recently described repeated measures correlation (Bakdash and Marusich, 2017) a statistical technique for determining the inter-individuals correlation for intra-subject multiple measurements (r(196) = 0.534, p<10e−15). These results strongly suggest that slow wave activity-related down-up transitions are directly linked to the increase in cortical functional connectivity during the prevalence of slow waves.
Figure 6. Population down-up transitions drive functional connectivity during slow wave activity.
(A) Functional connectivity of the BOLD signal using the cortex wide component of the ICA as covariable (CWCcov): i- Average of the correlation matrices (n = 8) of the mean BOLD signal in 96 cortical regions during slow wave activity using the pan-cortical component of the ICA as covariable (CWCcov, purple frame) or the flipped values of the same component as control (CWCinv, brown frame). ii- Cumulative distribution curve for the r-values of the correlations during slow wave activity using the cortex-wide component of the ICA as regressor (CWCcov), or similarly using the flipped values of the same component (CWCinv). The vertical gray line lies at the mean of the FDR values used as cutoff to identify significant correlations, ***p<0.001 (paired t-test, n = 15). iii- Plot of the number of significant correlations for each individual in both conditions **p<0.01. (B) Correlation between the number of significant pairs in the functional connectivity matrix and the number of down-up transitions. i- Correlation scheme. The number of down-up transitions was quantified for the respective time period (upper panel) and then correlated with the number of significant pairs obtained from the functional connectivity matrix (lower panel). ii- 5 out of 6 animals analyzed showed a significant positive correlation between number of down-up transitions and number of significant functionally connected pairs. Histogram subplots indicate the results of the permutation test. This analysis corroborates the Pearson correlation showing the r-scores of the five significant cases to lie beyond 5% of significance in the normal distribution built with 10000 surrogates of shuffled data.
Dual-cortical calcium photometry during slow wave activity shows increased signal correlation in down-up transitions
While the previous results demonstrated the role of down-up transitions for functional connectivity during slow wave activity, they are based on hemodynamic signals rather than on a direct measure of neural responses. The occurrence of slow waves in a spatially confined neural population can be detected using fiber photometry measuring fluorescent signals emitted by cells previously stained with a calcium indicator (Adelsberger et al., 2014; Grienberger et al., 2012). To corroborate our results on the neural population level, we conducted experiments employing simultaneous optic-fiber-based calcium recordings in somatosensory (S1) and visual (V1) cortex (Figure 7A,B). Slow wave related down-up transitions can be easily identified and quantified (Seamari et al., 2007) and have a duration of 2 s on average (Figure 7C). The amplitude of the cross-correlation between these two signals is significant (Figure 7D), with a delay matching the propagation time of the wave propagating from V1 to S1 ((n = 6) 51.2 mm/s ± 8.1 s.e.m). To probe if down-up transitions are underlying the increase of functional cortical connectivity in our slow wave activity data, we filtered the fluorescence and the BOLD signal at different frequency intervals before running the cross-correlation analysis (Figure 7B,D). Filtering the signal induced a partial abolishment of the slow waves in particular bandwidths (Figure 7C) without interfering with the correlation of both cortical signals in other frequencies. Therefore, one should expect a decrease in the signal correlation on those frequencies when the slow wave activity is abolished. Results of six recordings in three animals showed that the cross-correlation peaks significantly decrease in the frequency interval for which the down-up transitions were filtered out (Figure 7B,E). The cross-correlation values were significantly lower when data was filtered between 0.01 and 0.4 Hz (paired t-test: t(5) = 4.26, p=0.008), 0.2–0.6 Hz (t(5) = 7.99, p<0.001), and 0.3–0.7 (t(5) = 7.28, p<0.001). These results support the ones obtained previously with BOLD connectivity analyses at the cortical population level, demonstrating down-up transitions to be the main contributor to an increase of cortical functional connectivity during slow wave activity.
Figure 7. During slow wave activity, the correlation between distant optical recordings is driven by slow-wave-associated calcium waves.
(A) Schematic of fiber photometry in cortical areas S1 and V1. (B) Exemplary fluorescent signal trace recorded from V1 filtered at different band-stop intervals, excluding those particular intervals from the recordings. (C) Mean of the down-up transitions for a trace (∆f/f) at different band stop filters. It can be observed how the amplitude, length and size of the slow waves are modified at each filtered bandwidth. (D) Cross-correlation results of a single recording filtered at different band-stop intervals (color code is the same than in subplot B). (E) Average of the cross-correlation peak values (n = 6) at different band-stop intervals, ***p<0.001 (Paired t-test, n = 6).
Discussion
We demonstrated that different states of brain activity can modify properties of the functional connectome. ICA showed distinct functional networks for each of the investigated activity states. In the case of slow wave activity, a cortex-wide component was found in all animals, while for persistent activity, a clear compartmentalization of the cortical functional structures gave origin to well-determined functional networks, as the default mode or the auditory network. Notably, the relation of population activity to functional connectivity was not dependent on a specific anesthetic agent, suggesting that not the type of anesthesia used, but the induced activity state influenced resting-state networks. The functional connectivity analyses using cortical parcellation corroborated this concept, showing an increase in the connectivity r-scores as well as in the number of significant correlations during slow wave activity. Population activity dominated by slow waves is characterized by long periods of quiescence followed by short lapses of neural activity corresponding to a propagating wave (Stroh et al., 2013), as demonstrated by the simultaneous dual cortical calcium recordings. To rule out that the connectivity values observed during slow wave activity are due to the spurious consequence of an increase in power, we performed fALFF analysis and found significantly lower values for data obtained under slow wave activity, probably due to the long periods of quiescence observed in population slow wave activity.
Spatiotemporal network dynamics
The high degree of compartmentalization in persistent activity dominated networks, observed in the ICA can be corroborated by calculating the dependency of the distance in the r-scores between two areas. In a highly compartmentalized connectome, this dependency should be lower than in a system which is highly interconnected (Hagmann et al., 2008). Indeed, when correlating the r-scores and the Euclidean distance of the network’s pairs for persistent activity data, the correlation was significantly lower than for data obtained under slow wave activity. This finding suggests, that indeed, the functional architecture during persistent activity is dominated by specific compartmentalized network nodes, which are not necessarily spatially close, such as the default mode network. In sharp contrast, during slow wave activity, we observed a strong distance dependency, in line with a propagating wave of activity – a slow wave event – eventually recruiting large parts of the cortex. These propagating slow wave events might temporarily switch parts of the functional network from a hyperpolarized, rather quiescent down state into a depolarized up state of high excitability and thereby propagate over cortical areas (Sanchez-Vives et al., 2017; Sanchez-Vives and McCormick, 2000; Steriade et al., 1991).
Applying graph theory methods, we found that during persistent activity the functional network shows characteristic small world network dynamics, as they have been described previously for the sedated rat (D'Souza et al., 2014) and for the awake human brain (Bullmore and Sporns, 2009). During slow wave activity graph theory analysis demonstrated characteristics of highly interconnected random networks to be dominant, corroborating the idea of a lack of a spatially segregated functional connectivity signature in that state (Okun et al., 2019; Spoormaker et al., 2010).
One could argue that the difference in connectivity is reflecting a particular change in neural dynamics induced by a specific anesthetic regimen rather than presenting a correlate of a particular activity state, since it has been reported that functional connectivity patterns in the rodent brain change dramatically depending on the anesthetics used (Bukhari et al., 2017; Matsui et al., 2016; Paasonen et al., 2018; Wu et al., 2017). In order to rule out anesthesia-specific effects on connectivity, we demonstrated that decreasing the amount of isoflurane administrated to the animal led to a transition from slow wave to persistent activity. Indeed, population and functional connectivity dynamics observed under a low dose of isoflurane rather resembled the ones observed under medetomidine-induced persistent activity, than the ones observed during slow wave activity induced by high isoflurane anesthesia.
Recently, it is has been shown that spontaneous resting-state associated brain activity is non-random in the temporal domain and that this feature is well conserved for rodents and humans (Ma and Zhang, 2018). Interestingly, when we analyzed the temporal dynamics of connectivity patterns, we found that animals in persistent activity state, induced either by medetomidine or a low dose of isoflurane, showed rather temporally stable - persistent - connectivity dynamics (Destexhe et al., 2007; Sanchez-Vives and McCormick, 2000; Steriade et al., 1991). In contrast, animals in slow wave activity state showed fast, and temporally irregular transitions likely reflecting the bimodality of slow waves (Stroh et al., 2013). This suggests that individual specific events within slow wave activity could drive state-specific network signatures, while it has to be taken into account that the regularity of down-up transitions or slow wave events can depend on the overall physiological condition of the animal, such as the depth of anesthesia (Chauvette et al., 2011). Although, there is abundant evidence on the relevance of, for example, infra-slow electrical brain activity for resting state connectivity (Hiltunen et al., 2014; Krishnan et al., 2018; Mitra et al., 2018), there so far has been no evidence linking different population activity states of the brain to changes in functional network configuration directly.
When we used the cortex-wide component as a covariable in the connectivity analyses, the connectivity values decreased significantly. This component represents a direct correlate of slow wave activity in a local neural population (Schwalm et al., 2017) and is therefore evident for the occurrence of population down-up transitions. A direct demonstration of the involvement of down-up transitions in the functional connectivity maps comes from the significant correlation between the degree of connectivity and the amount of transitions in 4 of 5 animals. To provide evidence for this hypothesis, we conducted simultaneous fiber photometry alongside fMRI measurements during slow wave activity, as previously implemented (Schmid et al., 2016). When the component of down-up transitions identified in the fluorescence signal was filtered out, we indeed found a decrease in correlation of cortical activity. These findings provide additional evidence that population down-up transitions can drive functional connectivity in the cortex.
It has been suggested that the generation and propagation of slow waves is depending on changes in cortical excitability during sleep (Massimini et al., 2004) or under anesthesia (Ruiz-Mejias et al., 2011), even differentially affecting cortical layers (Capone et al., 2019). Disrupted slow wave propagation can be reflective of pathophysiological conditions such as Alzheimer’s disease (Busche et al., 2019). We therefore propose that distinct activity states of the brain, that is defined spatio-temporal dynamics of cortical excitability, drive the brain’s functional connectivity. The here investigated rather discrete states – persistent versus slow wave activity, are characterized by particular and well-defined network signatures. The understanding of network dynamics underlying respective functional network patterns will reduce the variability found in resting-state data and increase the translational value of resting-state fMRI studies performed in rodents.
Implications on resting state studies
Several studies have demonstrated that the functional state of the brain is dynamic over time, and that this is reflected in changes in functional connectivity (Belloy et al., 2018; Grandjean et al., 2017). These dynamic states are quantifiable, depend on ongoing activity and seem to show atypical dynamics in some neurological disorders (Gutierrez-Barragan et al., 2019). Degrees of wakefulness seem to play an important role in global functional connectivity (Turchi et al., 2018). One study showed that during resting-state recordings in humans, dynamic changes in functional connectivity occur depending on different wakefulness and sleep patterns (Tagliazucchi and Laufs, 2014). More recently, Laumann and colleagues showed that the dynamic variability over time in fMRI functional connectivity signatures can be explained by fluctuating sleep stages (Laumann et al., 2017).
Previous resting-state fMRI studies report that anesthetized animals show lower functional coupling than awake animals (Vincent et al., 2007; Yin et al., 2019). However, some studies show that default mode networks in rodents can be found even after the loss of consciousness (Liang et al., 2012; Lu et al., 2012), while others show a breakdown in the networks of resting state connectivity under anesthesia (Boveroux et al., 2010; Stamatakis et al., 2010). These apparent dissimilar results have been hypothesized to be dependent on the synchronization of infra-slow oscillatory activity and cross-frequency coupling (Hutchison et al., 2013). Our experiments demonstrate that the origin of these discrepancies may lie in the presence of different activity states of the brain. During persistent activity, compartmentalized patterns are detected, whilst during slow wave activity the degree of connectivity depends on the rate of slow waves generated in a particular period of time. Global signal removal has been shown to reduce connectivity for high isoflurane global synchrony conditions (Grandjean et al., 2014). This might indeed be because this technique is removing the underlying down-up transition related signals which are actually explaining a relevant part of the increased connectivity as our results show. Activity states of the brain as well as the rate of down-up transitions during slow wave activity in particular are dynamic and can be present under different experimental settings (Vyazovskiy et al., 2011). Here, we present data from strictly controlled conditions, in which the entire cortex seems to be engaged in either persistent or slow wave activity. However, in awake animals or during slow wave sleep, this might not be the case.
The presented results call for a brain state-informed analysis of functional connectivity in resting-state studies, both in humans and in animal models. Human subjects may transition between similar subtypes of these activity states when they transition between different levels of attention during an experiment or when they fall asleep (Stevner et al., 2019). Even more so, animals may react differently to the same anaesthetic regimen (Vyazovskiy et al., 2011) and therefore present a different underlying state of cortical activity while being subjected to the same anaesthesia condition. Similarly, it might be the case that at the beginning of an imaging experiment, an animal is in a state of persistent activity, and while the experiment progresses, shifts into a state of slow wave activity due to a change of underlying physiology or deepened anaesthesia over time. As our results show that this would lead to drastically different functional connectivity signatures, it is necessary to consider the particular activity state and global changes in cortical excitability before deriving conclusions about networks dynamics in any resting-state fMRI experiment.
Materials and methods
Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (Rattus norvegicus) | Lewis rat | Janvier labs | LEW/OrlRj | Female 160–200 gr |
Chemical compound, drug | PBS tabletts | gibco | 18912–014 | |
Chemical compound, drug | xylocaine | AstraZeneca | PUN080440 | 2% |
Chemical compound, drug | isoflurane | AbbVie | 8506 CHEBI:6015 | 1–1.55% |
Chemical compound, drug | Oregon-Green BAPTA1 AM | Invitrogen Thermo Fisher scientific |
O6807 | 1 mM |
Chemical compound, drug | Glucose | B. Brown | 2355740 | 10% |
Chemical compound, drug | Medetomidine hydrochloride | Dorbene vet. Zoetis | 08164–43 | 0.08 mg/kg/hr |
Chemical compound, drug | Carbomer | Vidisic | 74013T296/52-DE | 2 mg/gr |
Chemical compound, drug | NaCl | Fresenius Kabi France | 13KLP183 | 0.9% |
Chemical compound, drug | Agarose | Sigma-Aldrich | CAS: 9012-36-6 | 2% |
Software, algorithm | Brain Voyager 20.6 | Brain Innovation, Maastricht, Netherlands | ||
Software, algorithm | Matlab R2018a | (The Mathworks, Inc, Natick, MA, USA) | Codes available at https://github.com/Strohlab/connectivityelife copy archived athttps://github.com/elifesciences-publications/connectivityelife |
Animals
Experiments were performed on 25 adult female Lewis rats (>12 weeks old, 160–200 g), of which 15 were used for fMRI measurements, six were implanted with an optic fiber in the visual cortex for combined optic-fiber-based calcium recordings/fMRI measurements and four were used for optical recordings on the bench. Animals were housed under a 12 hr light–dark cycle and provided with food and water ad libitum. Animal husbandry and experimental manipulation were carried out according to animal welfare guidelines of the Johannes Gutenberg-University Mainz and were approved by the Landesuntersuchungsamt Rheinland-Pfalz, Koblenz, Germany (G14-1-040). fMRI data acquisition fMRI data acquisition for connectivity analyses was performed in two sessions for each animal. For recording slow wave activity data sets, animals were anesthetized with isoflurane (1.2–1.8% in 80% air and 20% oxygen). For recording persistent activity data, animals were sedated with medetomidine (zoetis, bolus injection of 0.04 mg/kg i.p. followed by continuous i.p. infusion of 0.08 mg/kg/hr). In a second group (low isoflurane group), animals were sedated with isoflurane 0.5–0.7% in order to keep the animals in persistent activity. The order of the recording sessions was randomly assigned to each animal. Both sessions were separated by at least 1 week. Measurements were performed on a 9.4 T small animal imaging system with a 0.7 T/m gradient system (Biospec 94/20, Bruker Biospin GmbH, Ettlingen, Germany). Each experimental protocol consisted of T1-weighted anatomical imaging (image size 175 × 175×80, FoV 26 × 29×28 mm, resolution 0.15 × 0.165×0.35 mm), 30 min of resting state T2*-weighted images acquired with a single-shot gradient echo EPI sequence (TR = 1.5 s, TE = 14 ms and FA 65°, 320 × 290 μm² spatial resolution, slice thickness 0.8 mm, 34 continuous slices, 1200 acquisitions in eight animals and TR = 1 s in another seven animals) and a high-resolution in-plane T2-MSME images of the 34 slices (TE = 12 ms, 100 × 100 μm² spatial resolution, image size 256 × 256, slice thickness 0.8 mm, 34 continuous slices). The same protocol was used in the combined recordings with optic-fiber-based calcium recordings. Body temperature and breathing rate of the animal were monitored and recorded during the entire experimental procedure using an SA instruments small animal MRI-compatible monitoring system (Small Animal Instruments, Inc, Stony Brook, NY).
fMRI analysis
Data preprocessing
General Linear Model (GLM) fMRI data processing was performed using Brain Voyager 20.6 for windows (Brain Innovation, Maastricht, Netherlands). Preprocessing steps included slice scan time correction, 3D motion correction and smoothing of the images, using cubic-spline interpolation method and trilinear/sinc interpolation for the 3D motion correction. Datasets were smoothed using a 0.8 mm FWHM Gaussian Kernel. The initial five images were discarded to include only signals that have reached steady state. T1 images were manually aligned to the rat atlas MRI template of Valdes-Hernandez et al. (Valdés-Hernández et al., 2011). T2*-weighted images and high-resolution in-plane T2*-weighted images were then manually aligned to these T1 previously aligned to the MRI template. The same preprocessing steps were performed for the scans combined with fiber photometry recordings.
Independent component analysis (ICA)
For ICA, the freely available software ‘Group ICA of fMRI Toolbox (GIFT)”, version 4.0b (http://mialab.mrn.org/software/gift/; RRID:SCR_001953) was used. Mask-calculation using the first image was employed and time-series were linearly detrended and converted to z-scores. For back-reconstruction, the ‘GICA’-algorithm (Calhoun et al., 2001) has been used and all results have been scaled by their z-scores. We used the Infomax-algorithm with regular stability analysis without autofill. Images for the ICA were motion corrected and smoothed as described above for each animal. The number of ICs to calculate for each animal was set to 20 and 25 for checking whether some components were subdivided into partial networks. We did not find different components for the networks of interests between both analyses; thus, we used the 20 components. Breathing rate and the signal form ventricles were used a regressors to avoid spurious components. Components that showed clusters outside the brain or a distinctive single slide activity were considered as noise and discarded. For visualization purposes, the z-scores of each animal were averaged and then plotted over the template using the Brain Voyager software (Figure 1). The regions found in the persistent activity ICA were taken as ROI for a connectivity analysis. nine regions were identified base in the connectivity patterns. The time course from each of the nine preselected nodes was extracted, and computed the Pearson correlation between every pair of the nodes to form a correlation matrix. The average signal of the ventricles and white matter was extracted and together with the breathing signal added as a covariable in the correlation analysis. This step resulted in a 9 × 9 matrix for each rat, where r-scores represented the resting state functional connectivity strength between each pair.
Connectivity analysis
Connectivity analyses were performed using custom Matlab scripts (The Mathworks, Inc, Natick, MA). After normalizing the BOLD-signal to its mean intensity, the cortical volume was parcellated in 96 regions of interest (ROIs) using the MRI template of Valdés-Hernández et al., 2011. The size of each region consisted in average on 2014 voxels. With an exponential decay distribution. 75% of the regions contained more than 300 voxels with a minimum of 102 voxels and a maximum of 10160. The mean signal for each area was calculated averaging the signal of each voxel. The average signal of the ventricles and white matter was extracted and together with the breathing signal added as a covariable in the correlations. In a second connectivity analysis (Figure 6), the time course of the cortex-wide component was added as a covariable, either in its original form or temporally reversed as a control. Then, partial correlation coefficients were calculated for each pair of ROIs. The significance cutoff for each pair of ROIs was calculated independently for each matrix (each individual) using false discovery rate (FDR) correction (mean of cuttof: 0.348 ± 0.045 s.e.m). The FDR was performed on each matrix using the function mafdr from matlab based on the method introduced by Storey, 2002. Thus, any value above the calculated FDR was considered as a significant connection between 2 ROIs.
fALFF analysis
The amplitude of low-frequency fluctuations (ALFF) of the resting-state fMRI signal is defined as the power of the signal within a certain frequency range and measures the intensity of regional spontaneous brain activity in humans (Zang et al., 2007) as a measure of local metabolism in a particular region. To reduce sensitivity to physiological noise, Zou et al., 2008 introduced fractional ALFF (fALFF), which is defined as the ratio of low-frequency amplitudes (0.01–0.08 Hz) to the amplitudes of the entire frequency range (0–0.25 Hz). Similarly, we conducted fALFF analysis using the ratio of 0.01–0.1 Hz for the entire frequency range 0–0.25 Hz. We calculated the fFALFF value for each voxel and then averaged those values for each ROI and compared the distribution of the 96 ROIs for both investigated activity states (slow wave activity and persistent activity).
Euclidean distance
The correlation between r-scores and the Euclidean distances between the ROIs were calculated between each pair of ROIs taking the average coordinates of the voxels belonging to each ROI as its centroid.
Graph theory analysis
Adjacency matrices were constructed starting from correlation matrices of the functional connectivity analysis, and after thresholding (threshold was given for each matrix based on the FDR correction previously calculated, see connectivity analysis above) were configured as Weighted Undirected Networks taking into account only the positively correlated values. This construction of matrix adjacency has been pointed as necessary when functional MRI datasets are analysed (Rubinov and Sporns, 2010). The adjacency matrices construction and the network measures calculated were all derived from the adaptation of the Complex Networks Analysis toolbox Functional Brain Connectivity for Matlab (Rubinov and Sporns, 2010). Network nodes in our case were ROIs, and networks links were the magnitudes of temporal correlations between ROIs, obtained by correlation coefficients calculated previously.
Matrix similarity analysis
Matrix similarity was calculated using the Frobenius Norm of the eigenvalues of each matrix. This calculation gives a measure of the distance between the two vectors resulting from the eigenvalues of the matrix. The closer to zero the obtained norm is, the closer are the vectors and therefore, the more similar are the matrices.
Calcium indicator injections and optic fiber placement
For surgical procedures including staining with fluorescent calcium indicator and optic fiber implantation, rats were anesthetized with isoflurane (Forene, Abbvie, Ludwigshafen, Germany), placed on a warming pad (37° C), and fixed in a stereotactic frame with ear and bite bars.
The skull was exposed, dried from blood and fluids, and leveled for precise stereotactic injections. Under a dissection microscope a small craniotomy was performed with a dental drill (Ultimate XL-F, NSK, Trier Germany, and VS1/4HP/005, Meisinger, Neuss, Germany). The fluorescent calcium sensitive dye Oregon Green 488 BAPTA-1AM (OGB-1, Invitrogen, Life Technologies, Carlsbad, CA) was prepared as described previously (Garaschuk et al., 2006), filtered, and injected into primary visual cortex (V1; −5.5 mm AP, +3.8 mm ML, −0.5,–0.7 and 0.9 mm DV) for the experiments combining fMRI and calcium photometry, and a second craniotomy and injection were performed in the primary somatosensory cortex front limb area (S1FL; 0 mm AP, +3.5 mm ML, −0.5,–0.7 and 0.9 mm DV;) for the bench experiments including optical recordings in the two cortical areas. After injection of the indicator, the pipette was held in place for 2 min before slowly retracting it from the tissue. After removing the cladding from the tip, an optic fiber with a diameter of 200 µm was inserted perpendicular to the dura above the OGB-1 stained area, typically at 300 µm and fixed to the skull with UV glue (Polytec, PT GmbH, Waldbrunn, Germany). A detailed description of these procedures can be found in Stroh et al., 2013 and Schwalm et al., 2017.
Fiber photometry calcium recordings
For optic-fiber-based photometry calcium recordings, custom-built setups were used (FOM and FOMII, npi Electronic Instruments, Tamm, Germany), as reported previously (Stroh, 2018; Schwalm et al., 2017). The light for excitation of the calcium indicator OGB-1 was delivered by a 650 mW LED with a nominal peak wavelength of 470 nm. LED power was controlled by an adjustable current source. The light beam was focused by means of a fixed focus collimator into one end of a multimode optic fiber which was connected to the system by an SMA connector. The recorded fluorescence signals were digitized with a sampling frequency of 2 kHz using a multifunction I/O data acquisition interface (Power1401, Cambridge Electronic Design, Cambridge, UK) and its corresponding acquisition software (Spike2).
Calcium recordings analysis
Data was read into Matlab (The Mathworks, Inc, Natick, MA, USA) and downsampled from 2 kHz to 1 kHz by averaging two adjacent values. The baseline of the fluorescence signal was determined and corrected by using the Matlab function msbackadj estimating the baseline within multiple shifted windows of 2500 datapoints width, regressing varying baseline values to the window’s datapoints using a spline approximation, then adjusting the baseline in the peak range of the fluorescent signal. This method provides a correction for low-frequency drifts while avoiding contributions of signal-of-interest events. We employed a procedure based on exponential moving average (EMA) filters, originally established to identify slow waves in electrophysiological recordings (Seamari et al., 2007). Onsets of slow calcium waves were defined as signal timepoints exceeding the threshold (70%), termination of calcium slow waves was defined as signal timepoints dropping below 50% of the threshold value. Further, the detected activity was post-processed in the following order: 1) calcium waves separated by a time-interval below 500 ms were interpreted as one wave; 2) calcium waves with a duration of less than 300 ms were discarded; 3) activity not reaching 90% of the histogram-based cumulative signal intensity was discarded. The correlation analysis with the fMRI connectivity values was performed taking sub-traces of 180 s. This is equivalent of 120 data points in the BOLD signal, above the minimum of 100 points that has been shown to be necessary in the analysis of dynamic functional connectivity between 0.01 and 0.2 Hz with an overlap of 66% (Leonardi and Van De Ville, 2015). To avoid spurious results in the correlation analysis given by the overlap of the collected data points, we performed a permutation test for each individual dataset, creating 10,000 data surrogates shuffling the connectivity indexes with the slow wave events in each subject. The normal distribution of the obtained r-scores represent the chance level of a spurious correlation and the mean of the distribution, the degree of intra-dependence. Thus, the obtained r-score is considered as significant when it falls into the 5% of the extremes of the distribution. The repeated measures correlation was performed using R software for statistical computing (R Development Core Team, 2010) using the rmcorr package (Bakdash and Marusich, 2017).
Statistics
All statistical analysis were conducted using the statistical and machine learning toolbox of Matlab (The Mathworks, Inc, Natick, MA). Statistical measures were calculated in the parametric space, when attributes of data distributions, normality and homoscedasticity criteria allowed it. Statistical analysis comparing both conditions (slow wave and persistent activity) were conducted using paired t-test (same animals, different conditions) and plotted using the boxplot function over the data points distribution. Values are reported as mean ± SEM for normally distributed data.
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting files. All source data files are available on Dryad Digital repository (https://doi.org/10.5061/dryad.vmcvdncqk).
All custom Matlab codes used in these analyses are available at https://github.com/Strohlab/connectivityelife (Aedo-Jury and Stroh, 2020; copy archived at https://github.com/elifesciences-publications/connectivityelife).
Acknowledgements
This work was supported by the DFG (SPP 1665: Resolving and manipulating neuronal networks in the mammalian brain - from correlative to causal analysis; CRC1193: Neurobiology of resilience to stress-related mental dysfunction: from understanding mechanisms to promoting preventions) and the Focus Program translational Neurosciences (FTN-Mainz). Authors thank Nuse Afahaene for excellent technical assistance and Eduardo Rosales Jubal for support in the Graph theory analyses.
Funding Statement
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Contributor Information
Felipe Aedo-Jury, Email: felipe.aedo@lir-mainz.de.
Albrecht Stroh, Email: albrecht.stroh@unimedizin-mainz.de.
Jan-Marino Ramirez, Seattle Children's Research Institute, United States.
Christian Büchel, University Medical Center Hamburg-Eppendorf, Germany.
Funding Information
This paper was supported by the following grants:
Deutsche Forschungsgemeinschaft SFB 1193 to Albrecht Stroh.
Deutsche Forschungsgemeinschaft SPP 1665 to Albrecht Stroh.
Additional information
Competing interests
No competing interests declared.
Author contributions
Conceptualization, Data curation, Writing.
Conceptualization, Formal analysis, Writing.
Data curation, Software, Writing - review and editing.
Conceptualization, Funding acquisition, Writing.
Ethics
Animal experimentation: Animal husbandry and experimental manipulation were carried out according to animal welfare guidelines of the Johannes Gutenberg-University Mainz and were approved by the Landesuntersuchungsamt Rheinland-Pfalz, Koblenz, Germany. (G14-1-040).
Additional files
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files. All source data files are available on Dryad Digital repository (https://doi.org/10.5061/dryad.vmcvdncqk). All custom Matlab codes used in these analyses are available at https://github.com/Strohlab/connectivityelife (copy archived at https://github.com/elifesciences-publications/connectivityelife; Aedo-Jury & Stroh, 2020).
The following dataset was generated:
Aedo-Jury F, Schwalm M, Hamzehpour L, Stroh A. 2020. Brain states govern the spatio-temporal dynamics of resting-state functional connectivity. Dryad Digital Repository.
References
- Adelsberger H, Grienberger C, Stroh A, Konnerth A. In vivo calcium recordings and channelrhodopsin-2 activation through an optical fiber. Cold Spring Harbor Protocols. 2014;2014:pdb.prot084145. doi: 10.1101/pdb.prot084145. [DOI] [PubMed] [Google Scholar]
- Aedo-Jury F, Stroh A. Brain states govern the spatio-temporal dynamics of resting-state functional connectivity. 1.0Github. 2020 doi: 10.7554/eLife.53186. https://github.com/Strohlab/connectivityelife [DOI] [PMC free article] [PubMed]
- Bakdash JZ, Marusich LR. Repeated measures correlation. Frontiers in Psychology. 2017;8:456. doi: 10.3389/fpsyg.2017.00456. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barth AL, Poulet JF. Experimental evidence for sparse firing in the neocortex. Trends in Neurosciences. 2012;35:345–355. doi: 10.1016/j.tins.2012.03.008. [DOI] [PubMed] [Google Scholar]
- Becerra L, Pendse G, Chang PC, Bishop J, Borsook D. Robust reproducible resting state networks in the awake rodent brain. PLOS ONE. 2011;6:e25701. doi: 10.1371/journal.pone.0025701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Belloy ME, Naeyaert M, Abbas A, Shah D, Vanreusel V, van Audekerke J, Keilholz SD, Keliris GA, Van der Linden A, Verhoye M. Dynamic resting state fMRI analysis in mice reveals a set of Quasi-Periodic patterns and illustrates their relationship with the global signal. NeuroImage. 2018;180:463–484. doi: 10.1016/j.neuroimage.2018.01.075. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boveroux P, Vanhaudenhuyse A, Bruno MA, Noirhomme Q, Lauwick S, Luxen A, Degueldre C, Plenevaux A, Schnakers C, Phillips C, Brichant JF, Bonhomme V, Maquet P, Greicius MD, Laureys S, Boly M. Breakdown of within- and between-network resting state functional magnetic resonance imaging connectivity during propofol-induced loss of consciousness. Anesthesiology. 2010;113:1038–1053. doi: 10.1097/ALN.0b013e3181f697f5. [DOI] [PubMed] [Google Scholar]
- Buckner RL, Snyder AZ, Shannon BJ, LaRossa G, Sachs R, Fotenos AF, Sheline YI, Klunk WE, Mathis CA, Morris JC, Mintun MA. Molecular, structural, and functional characterization of Alzheimer's disease: evidence for a relationship between default activity, amyloid, and memory. Journal of Neuroscience. 2005;25:7709–7717. doi: 10.1523/JNEUROSCI.2177-05.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bukhari Q, Schroeter A, Cole DM, Rudin M. Resting state fMRI in mice reveals anesthesia specific signatures of brain functional networks and their interactions. Frontiers in Neural Circuits. 2017;11:5. doi: 10.3389/fncir.2017.00005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bukhari Q, Schroeter A, Rudin M. Increasing isoflurane dose reduces homotopic correlation and functional segregation of brain networks in mice as revealed by resting-state fMRI. Scientific Reports. 2018;8:10591. doi: 10.1038/s41598-018-28766-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bullmore E, Sporns O. Complex brain networks: graph theoretical analysis of structural and functional systems. Nature Reviews Neuroscience. 2009;10:186–198. doi: 10.1038/nrn2575. [DOI] [PubMed] [Google Scholar]
- Busche MA, Grienberger C, Keskin AD, Song B, Neumann U, Staufenbiel M, Förstl H, Konnerth A. Decreased amyloid-β and increased neuronal hyperactivity by immunotherapy in Alzheimer's models. Nature Neuroscience. 2015;18:1725–1727. doi: 10.1038/nn.4163. [DOI] [PubMed] [Google Scholar]
- Busche MA, Wegmann S, Dujardin S, Commins C, Schiantarelli J, Klickstein N, Kamath TV, Carlson GA, Nelken I, Hyman BT. Tau impairs neural circuits, dominating amyloid-β effects, in alzheimer models in vivo. Nature Neuroscience. 2019;22:57–64. doi: 10.1038/s41593-018-0289-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Calhoun VD, Adali T, Pearlson GD, Pekar JJ. A method for making group inferences from functional MRI data using independent component analysis. Human Brain Mapping. 2001;14:140–151. doi: 10.1002/hbm.1048. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Capone C, Rebollo B, Muñoz A, Illa X, Del Giudice P, Sanchez-Vives MV, Mattia M. Slow waves in cortical slices: how spontaneous activity is shaped by laminar structure. Cerebral Cortex. 2019;29:319–335. doi: 10.1093/cercor/bhx326. [DOI] [PubMed] [Google Scholar]
- Chang C, Leopold DA, Schölvinck ML, Mandelkow H, Picchioni D, Liu X, Ye FQ, Turchi JN, Duyn JH. Tracking brain arousal fluctuations with fMRI. PNAS. 2016;113:4518–4523. doi: 10.1073/pnas.1520613113. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chauvette S, Crochet S, Volgushev M, Timofeev I. Properties of slow oscillation during slow-wave sleep and anesthesia in cats. Journal of Neuroscience. 2011;31:14998–15008. doi: 10.1523/JNEUROSCI.2339-11.2011. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Constantinople CM, Bruno RM. Effects and mechanisms of wakefulness on local cortical networks. Neuron. 2011;69:1061–1068. doi: 10.1016/j.neuron.2011.02.040. [DOI] [PMC free article] [PubMed] [Google Scholar]
- D'Souza DV, Jonckers E, Bruns A, Künnecke B, von Kienlin M, Van der Linden A, Mueggler T, Verhoye M. Preserved modular network organization in the sedated rat brain. PLOS ONE. 2014;9:e106156. doi: 10.1371/journal.pone.0106156. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deco G, Jirsa VK, McIntosh AR. Resting brains never rest: computational insights into potential cognitive architectures. Trends in Neurosciences. 2013;36:268–274. doi: 10.1016/j.tins.2013.03.001. [DOI] [PubMed] [Google Scholar]
- Destexhe A, Hughes SW, Rudolph M, Crunelli V. Are corticothalamic 'up' states fragments of wakefulness? Trends in Neurosciences. 2007;30:334–342. doi: 10.1016/j.tins.2007.04.006. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Finn ES, Scheinost D, Finn DM, Shen X, Papademetris X, Constable RT. Can brain state be manipulated to emphasize individual differences in functional connectivity? NeuroImage. 2017;160:140–151. doi: 10.1016/j.neuroimage.2017.03.064. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox MD, Raichle ME. Spontaneous fluctuations in brain activity observed with functional magnetic resonance imaging. Nature Reviews Neuroscience. 2007;8:700–711. doi: 10.1038/nrn2201. [DOI] [PubMed] [Google Scholar]
- Garaschuk O, Milos RI, Konnerth A. Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo. Nature Protocols. 2006;1:380–386. doi: 10.1038/nprot.2006.58. [DOI] [PubMed] [Google Scholar]
- Gozzi A, Schwarz AJ. Large-scale functional connectivity networks in the rodent brain. NeuroImage. 2016;127:496–509. doi: 10.1016/j.neuroimage.2015.12.017. [DOI] [PubMed] [Google Scholar]
- Grandjean J, Schroeter A, Batata I, Rudin M. Optimization of anesthesia protocol for resting-state fMRI in mice based on differential effects of anesthetics on functional connectivity patterns. NeuroImage. 2014;102 Pt 2:838–847. doi: 10.1016/j.neuroimage.2014.08.043. [DOI] [PubMed] [Google Scholar]
- Grandjean J, Preti MG, Bolton TAW, Buerge M, Seifritz E, Pryce CR, Van De Ville D, Rudin M. Dynamic reorganization of intrinsic functional networks in the mouse brain. NeuroImage. 2017;152:497–508. doi: 10.1016/j.neuroimage.2017.03.026. [DOI] [PubMed] [Google Scholar]
- Grienberger C, Adelsberger H, Stroh A, Milos RI, Garaschuk O, Schierloh A, Nelken I, Konnerth A. Sound-evoked network calcium transients in mouse auditory cortex in vivo. The Journal of Physiology. 2012;590:899–918. doi: 10.1113/jphysiol.2011.222513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guo K, Benson PJ, Blakemore C. Pattern motion is present in V1 of awake but not anaesthetized monkeys. European Journal of Neuroscience. 2004;19:1055–1066. doi: 10.1111/j.1460-9568.2004.03212.x. [DOI] [PubMed] [Google Scholar]
- Gutierrez-Barragan D, Basson MA, Panzeri S, Gozzi A. Infraslow state fluctuations govern spontaneous fMRI network dynamics. Current Biology. 2019;29:2295–2306. doi: 10.1016/j.cub.2019.06.017. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hagmann P, Cammoun L, Gigandet X, Meuli R, Honey CJ, Wedeen VJ, Sporns O. Mapping the structural core of human cerebral cortex. PLOS Biology. 2008;6:e159. doi: 10.1371/journal.pbio.0060159. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hiltunen T, Kantola J, Abou Elseoud A, Lepola P, Suominen K, Starck T, Nikkinen J, Remes J, Tervonen O, Palva S, Kiviniemi V, Palva JM. Infra-slow EEG fluctuations are correlated with resting-state network dynamics in fMRI. Journal of Neuroscience. 2014;34:356–362. doi: 10.1523/JNEUROSCI.0276-13.2014. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hsu LM, Liang X, Gu H, Brynildsen JK, Stark JA, Ash JA, Lin CP, Lu H, Rapp PR, Stein EA, Yang Y. Constituents and functional implications of the rat default mode network. PNAS. 2016;113:E4541–E4547. doi: 10.1073/pnas.1601485113. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hutchison RM, Womelsdorf T, Allen EA, Bandettini PA, Calhoun VD, Corbetta M, Della Penna S, Duyn JH, Glover GH, Gonzalez-Castillo J, Handwerker DA, Keilholz S, Kiviniemi V, Leopold DA, de Pasquale F, Sporns O, Walter M, Chang C. Dynamic functional connectivity: promise, issues, and interpretations. NeuroImage. 2013;80:360–378. doi: 10.1016/j.neuroimage.2013.05.079. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hutchison RM, Hutchison M, Manning KY, Menon RS, Everling S. Isoflurane induces dose-dependent alterations in the cortical connectivity profiles and dynamic properties of the brain's functional architecture. Human Brain Mapping. 2014;35:5754–5775. doi: 10.1002/hbm.22583. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jonckers E, Van Audekerke J, De Visscher G, Van der Linden A, Verhoye M. Functional connectivity fMRI of the rodent brain: comparison of functional connectivity networks in rat and mouse. PLOS ONE. 2011;6:e18876. doi: 10.1371/journal.pone.0018876. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kalthoff D, Po C, Wiedermann D, Hoehn M. Reliability and spatial specificity of rat brain sensorimotor functional connectivity networks are superior under sedation compared with general anesthesia. NMR in Biomedicine. 2013;26:638–650. doi: 10.1002/nbm.2908. [DOI] [PubMed] [Google Scholar]
- Krishnan GP, González OC, Bazhenov M. Origin of slow spontaneous resting-state neuronal fluctuations in brain networks. PNAS. 2018;115:6858–6863. doi: 10.1073/pnas.1715841115. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Latora V, Marchiori M. Efficient behavior of small-world networks. Physical Review Letters. 2001;87:198701. doi: 10.1103/PhysRevLett.87.198701. [DOI] [PubMed] [Google Scholar]
- Laumann TO, Snyder AZ, Mitra A, Gordon EM, Gratton C, Adeyemo B, Gilmore AW, Nelson SM, Berg JJ, Greene DJ, McCarthy JE, Tagliazucchi E, Laufs H, Schlaggar BL, Dosenbach NUF, Petersen SE. On the stability of BOLD fMRI correlations. Cerebral Cortex. 2017;27:4719–4732. doi: 10.1093/cercor/bhw265. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leonardi N, Van De Ville D. On spurious and real fluctuations of dynamic functional connectivity during rest. NeuroImage. 2015;104:430–436. doi: 10.1016/j.neuroimage.2014.09.007. [DOI] [PubMed] [Google Scholar]
- Lewis CM, Baldassarre A, Committeri G, Romani GL, Corbetta M. Learning sculpts the spontaneous activity of the resting human brain. PNAS. 2009;106:17558–17563. doi: 10.1073/pnas.0902455106. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liang Z, King J, Zhang N. Intrinsic organization of the anesthetized brain. Journal of Neuroscience. 2012;32:10183–10191. doi: 10.1523/JNEUROSCI.1020-12.2012. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liang Z, Li T, King J, Zhang N. Mapping thalamocortical networks in rat brain using resting-state functional connectivity. NeuroImage. 2013;83:237–244. doi: 10.1016/j.neuroimage.2013.06.029. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liu X, Zhu XH, Zhang Y, Chen W. Neural origin of spontaneous hemodynamic fluctuations in rats under burst-suppression anesthesia condition. Cerebral Cortex. 2011;21:374–384. doi: 10.1093/cercor/bhq105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liu X, Zhu XH, Zhang Y, Chen W. The change of functional connectivity specificity in rats under various anesthesia levels and its neural origin. Brain Topography. 2013;26:363–377. doi: 10.1007/s10548-012-0267-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lu H, Zou Q, Gu H, Raichle ME, Stein EA, Yang Y. Rat brains also have a default mode network. PNAS. 2012;109:3979–3984. doi: 10.1073/pnas.1200506109. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ma Z, Zhang N. Temporal transitions of spontaneous brain activity. eLife. 2018;7:e33562. doi: 10.7554/eLife.33562. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Massimini M, Huber R, Ferrarelli F, Hill S, Tononi G. The sleep slow oscillation as a traveling wave. Journal of Neuroscience. 2004;24:6862–6870. doi: 10.1523/JNEUROSCI.1318-04.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsui T, Murakami T, Ohki K. Transient neuronal coactivations embedded in globally propagating waves underlie resting-state functional connectivity. PNAS. 2016;113:6556–6561. doi: 10.1073/pnas.1521299113. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitra A, Snyder AZ, Tagliazucchi E, Laufs H, Raichle ME. Propagated infra-slow intrinsic brain activity reorganizes across wake and slow wave sleep. eLife. 2015;4:e10781. doi: 10.7554/eLife.10781. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitra A, Kraft A, Wright P, Acland B, Snyder AZ, Rosenthal Z, Czerniewski L, Bauer A, Snyder L, Culver J, Lee JM, Raichle ME. Spontaneous Infra-slow brain activity has unique spatiotemporal dynamics and laminar structure. Neuron. 2018;98:297–305. doi: 10.1016/j.neuron.2018.03.015. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitra A, Raichle ME. How networks communicate: propagation patterns in spontaneous brain activity. Philosophical Transactions of the Royal Society B: Biological Sciences. 2016;371:20150546. doi: 10.1098/rstb.2015.0546. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nasrallah FA, Lew SK, Low AS, Chuang KH. Neural correlate of resting-state functional connectivity under α2 adrenergic receptor agonist, medetomidine. NeuroImage. 2014;84:27–34. doi: 10.1016/j.neuroimage.2013.08.004. [DOI] [PubMed] [Google Scholar]
- Nir Y, Staba RJ, Andrillon T, Vyazovskiy VV, Cirelli C, Fried I, Tononi G. Regional slow waves and spindles in human sleep. Neuron. 2011;70:153–169. doi: 10.1016/j.neuron.2011.02.043. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Okun M, Steinmetz NA, Lak A, Dervinis M, Harris KD. Distinct structure of cortical population activity on fast and infraslow timescales. Cerebral Cortex. 2019;29:2196–2210. doi: 10.1093/cercor/bhz023. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paasonen J, Stenroos P, Salo RA, Kiviniemi V, Gröhn O. Functional connectivity under six anesthesia protocols and the awake condition in rat brain. NeuroImage. 2018;172:9–20. doi: 10.1016/j.neuroimage.2018.01.014. [DOI] [PubMed] [Google Scholar]
- Pachitariu M, Lyamzin DR, Sahani M, Lesica NA. State-dependent population coding in primary auditory cortex. Journal of Neuroscience. 2015;35:2058–2073. doi: 10.1523/JNEUROSCI.3318-14.2015. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pais-Roldán P, Takahashi K, Sobczak F, Chen Y, Zhao X, Zeng H, Jiang Y, Yu X. Indexing brain state-dependent pupil dynamics with simultaneous fMRI and optical fiber calcium recording. PNAS. 2020;117:6875–6882. doi: 10.1073/pnas.1909937117. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pawela CP, Biswal BB, Cho YR, Kao DS, Li R, Jones SR, Schulte ML, Matloub HS, Hudetz AG, Hyde JS. Resting-state functional connectivity of the rat brain. Magnetic Resonance in Medicine. 2008;59:1021–1029. doi: 10.1002/mrm.21524. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Petersen CC, Hahn TT, Mehta M, Grinvald A, Sakmann B. Interaction of sensory responses with spontaneous depolarization in layer 2/3 barrel cortex. PNAS. 2003;100:13638–13643. doi: 10.1073/pnas.2235811100. [DOI] [PMC free article] [PubMed] [Google Scholar]
- R Development Core Team . Vienna, Austria: R Foundation for Statistical Computing; 2010. http://www.r-project.org [Google Scholar]
- Raichle ME. The restless brain. Brain Connectivity. 2011;1:3–12. doi: 10.1089/brain.2011.0019. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raichle ME. The restless brain: how intrinsic activity organizes brain function. Philosophical Transactions of the Royal Society B: Biological Sciences. 2015;370:20140172. doi: 10.1098/rstb.2014.0172. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubinov M, Sporns O. Complex network measures of brain connectivity: uses and interpretations. NeuroImage. 2010;52:1059–1069. doi: 10.1016/j.neuroimage.2009.10.003. [DOI] [PubMed] [Google Scholar]
- Ruiz-Mejias M, Ciria-Suarez L, Mattia M, Sanchez-Vives MV. Slow and fast rhythms generated in the cerebral cortex of the anesthetized mouse. Journal of Neurophysiology. 2011;106:2910–2921. doi: 10.1152/jn.00440.2011. [DOI] [PubMed] [Google Scholar]
- Sanchez-Vives MV, Massimini M, Mattia M. Shaping the default activity pattern of the cortical network. Neuron. 2017;94:993–1001. doi: 10.1016/j.neuron.2017.05.015. [DOI] [PubMed] [Google Scholar]
- Sanchez-Vives MV, McCormick DA. Cellular and network mechanisms of rhythmic recurrent activity in neocortex. Nature Neuroscience. 2000;3:1027–1034. doi: 10.1038/79848. [DOI] [PubMed] [Google Scholar]
- Schmid F, Wachsmuth L, Schwalm M, Prouvot PH, Jubal ER, Fois C, Pramanik G, Zimmer C, Faber C, Stroh A. Assessing sensory versus optogenetic network activation by combining (o)fMRI with optical Ca2+ recordings. Journal of Cerebral Blood Flow & Metabolism. 2016;36:1885–1900. doi: 10.1177/0271678X15619428. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwalm M, Schmid F, Wachsmuth L, Backhaus H, Kronfeld A, Aedo Jury F, Prouvot PH, Fois C, Albers F, van Alst T, Faber C, Stroh A. Cortex-wide BOLD fMRI activity reflects locally-recorded slow oscillation-associated calcium waves. eLife. 2017;6:e27602. doi: 10.7554/eLife.27602. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwarz AJ, Gozzi A, Bifone A. Community structure and modularity in networks of correlated brain activity. Magnetic Resonance Imaging. 2008;26:914–920. doi: 10.1016/j.mri.2008.01.048. [DOI] [PubMed] [Google Scholar]
- Seamari Y, Narváez JA, Vico FJ, Lobo D, Sanchez-Vives MV. Robust off- and online separation of intracellularly recorded up and down cortical states. PLOS ONE. 2007;2:e888. doi: 10.1371/journal.pone.0000888. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shin TJ, Cho D, Ham J, Choi DH, Kim S, Jeong S, Kim HI, Kim JG, Lee B. Changes in thalamo-frontal interaction under different levels of anesthesia in rats. Neuroscience Letters. 2016;627:18–23. doi: 10.1016/j.neulet.2016.05.045. [DOI] [PubMed] [Google Scholar]
- Spoormaker VI, Schröter MS, Gleiser PM, Andrade KC, Dresler M, Wehrle R, Sämann PG, Czisch M. Development of a large-scale functional brain network during human non-rapid eye movement sleep. Journal of Neuroscience. 2010;30:11379–11387. doi: 10.1523/JNEUROSCI.2015-10.2010. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stamatakis EA, Adapa RM, Absalom AR, Menon DK. Changes in resting neural connectivity during propofol sedation. PLOS ONE. 2010;5:e14224. doi: 10.1371/journal.pone.0014224. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Staresina BP, Alink A, Kriegeskorte N, Henson RN. Awake reactivation predicts memory in humans. PNAS. 2013;110:21159–21164. doi: 10.1073/pnas.1311989110. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steriade M, Dossi RC, Nuñez A. Network modulation of a slow intrinsic oscillation of cat thalamocortical neurons implicated in sleep Delta waves: cortically induced synchronization and brainstem cholinergic suppression. The Journal of Neuroscience. 1991;11:3200–3217. doi: 10.1523/JNEUROSCI.11-10-03200.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steriade M, Timofeev I, Grenier F. Natural waking and sleep states: a view from inside neocortical neurons. Journal of Neurophysiology. 2001;85:1969–1985. doi: 10.1152/jn.2001.85.5.1969. [DOI] [PubMed] [Google Scholar]
- Stevner ABA, Vidaurre D, Cabral J, Rapuano K, Nielsen SFV, Tagliazucchi E, Laufs H, Vuust P, Deco G, Woolrich MW, Van Someren E, Kringelbach ML. Discovery of key whole-brain transitions and dynamics during human wakefulness and non-REM sleep. Nature Communications. 2019;10:1035. doi: 10.1038/s41467-019-08934-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Storey JD. A direct approach to false discovery rates. Journal of the Royal Statistical Society: Series B. 2002;64:479–498. doi: 10.1111/1467-9868.00346. [DOI] [Google Scholar]
- Stroh A, Adelsberger H, Groh A, Rühlmann C, Fischer S, Schierloh A, Deisseroth K, Konnerth A. Making waves: initiation and propagation of corticothalamic Ca2+ waves in vivo. Neuron. 2013;77:1136–1150. doi: 10.1016/j.neuron.2013.01.031. [DOI] [PubMed] [Google Scholar]
- Stroh AE. Optogenetics: A Roadmap. Humana Press; 2018. [DOI] [Google Scholar]
- Tagliazucchi E, Laufs H. Decoding wakefulness levels from typical fMRI resting-state data reveals reliable drifts between wakefulness and sleep. Neuron. 2014;82:695–708. doi: 10.1016/j.neuron.2014.03.020. [DOI] [PubMed] [Google Scholar]
- Turchi J, Chang C, Ye FQ, Russ BE, Yu DK, Cortes CR, Monosov IE, Duyn JH, Leopold DA. The basal forebrain regulates global Resting-State fMRI fluctuations. Neuron. 2018;97:940–952. doi: 10.1016/j.neuron.2018.01.032. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Valdés-Hernández PA, Sumiyoshi A, Nonaka H, Haga R, Aubert-Vásquez E, Ogawa T, Iturria-Medina Y, Riera JJ, Kawashima R. An in vivo MRI template set for morphometry, tissue segmentation, and fMRI localization in rats. Frontiers in Neuroinformatics. 2011;5:26. doi: 10.3389/fninf.2011.00026. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vincent JL, Patel GH, Fox MD, Snyder AZ, Baker JT, Van Essen DC, Zempel JM, Snyder LH, Corbetta M, Raichle ME. Intrinsic functional architecture in the anaesthetized monkey brain. Nature. 2007;447:83–86. doi: 10.1038/nature05758. [DOI] [PubMed] [Google Scholar]
- Vyazovskiy VV, Olcese U, Hanlon EC, Nir Y, Cirelli C, Tononi G. Local sleep in awake rats. Nature. 2011;472:443–447. doi: 10.1038/nature10009. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watts DJ, Strogatz SH. Collective dynamics of ‘small-world’ networks. Nature. 1998;393:440–442. doi: 10.1038/30918. [DOI] [PubMed] [Google Scholar]
- Woodward ND, Rogers B, Heckers S. Functional resting-state networks are differentially affected in schizophrenia. Schizophrenia Research. 2011;130:86–93. doi: 10.1016/j.schres.2011.03.010. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wu T, Grandjean J, Bosshard SC, Rudin M, Reutens D, Jiang T. Altered regional connectivity reflecting effects of different anaesthesia protocols in the mouse brain. NeuroImage. 2017;149:190–199. doi: 10.1016/j.neuroimage.2017.01.074. [DOI] [PubMed] [Google Scholar]
- Yin D, Zhang Z, Wang Z, Zeljic K, Lv Q, Cai D, Wang Y, Wang Z. Brain map of intrinsic functional flexibility in anesthetized monkeys and awake humans. Frontiers in Neuroscience. 2019;13:174. doi: 10.3389/fnins.2019.00174. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zang YF, He Y, Zhu CZ, Cao QJ, Sui MQ, Liang M, Tian LX, Jiang TZ, Wang YF. Altered baseline brain activity in children with ADHD revealed by resting-state functional MRI. Brain & Development. 2007;29:83–91. doi: 10.1016/j.braindev.2006.07.002. [DOI] [PubMed] [Google Scholar]
- Zhang Z, Cai DC, Wang Z, Zeljic K, Wang Z, Wang Y. Isoflurane-Induced burst suppression increases intrinsic functional connectivity of the monkey brain. Frontiers in Neuroscience. 2019;13:296. doi: 10.3389/fnins.2019.00296. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zou QH, Zhu CZ, Yang Y, Zuo XN, Long XY, Cao QJ, Wang YF, Zang YF. An improved approach to detection of amplitude of low-frequency fluctuation (ALFF) for resting-state fMRI: fractional ALFF. Journal of Neuroscience Methods. 2008;172:137–141. doi: 10.1016/j.jneumeth.2008.04.012. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zucca S, D'Urso G, Pasquale V, Vecchia D, Pica G, Bovetti S, Moretti C, Varani S, Molano-Mazón M, Chiappalone M, Panzeri S, Fellin T. An inhibitory gate for state transition in cortex. eLife. 2017;6:e26177. doi: 10.7554/eLife.26177. [DOI] [PMC free article] [PubMed] [Google Scholar]