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. 2020 Jun 1;10(16):7193–7210. doi: 10.7150/thno.44877

Figure 5.

Figure 5

ERRα regulates CPT1C expression and activates CPT1C transcription directly. (A) Expression of CPT1C mRNA in PANC-1 and MDAMB-231 cells after modulating ERRα expression and activity with the pENTER-ERRα plasmid and an agonist β-E2 (20 nM), as well as ERRα siRNA and an inhibitor XCT790 (20 μM). Data are mean ± SD (n = 6). (B) Western blot analysis ERRα and CPT1C after perturbation of ERRα. Data are mean ± SD (n = 3). (C) MDA-MB-231 cell lines stained for CPT1C. (D) Luciferase reporter gene assays were conducted in HEK-293T cells to compare transcriptional activities among plasmids within different lengths of CPT1C promoter regions or mutated ERRE sequences. MUT-G-CPT1C: ERRE-2-GREEN were mutated. MUT-R-CPT1C: ERRE-1-RED were mutated. MUT-Y-CPT1C: ERRE-3-YELLOW were mutated. Data are mean ± SD (n = 5). (E) MDA-MB-231 cells were treated with the pENTER-ERRα plasmid for 48 h, and ChIP analysis was performed. The DNA samples from the precipitation were amplified with PCR.