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. 2020 Jun 20;23(7):101291. doi: 10.1016/j.isci.2020.101291

Figure 4.

Figure 4

Candidate lincRNA Overexpression Impacts Cell Cycle Progression

(A) Histogram of the mean cell expression of all considered loci in mESCs. Lines indicate the expression of lincCC1 and lincCC2.

(B–D) (B) Ratio between the nuclear and cytoplasmic expression (log10) for two cytosolic mRNAs (PolII and Gapdh), two nuclearly retained lincRNAs (Malat1 and Neat1), and lincCC1 and lincCC2. Each point corresponds to the results for one biological replicate. Relative (C) lincCC1 and (D) lincCC2 expression 72 h after co-transfection of Cas9-VP160 with scramble-, gRNA1-, or gRNA2-expressing constructs for three independent biological replicates.

(E) Percentage of mESCs at G1, S, and G2/M stages of the cell cycle 72 h post transfection of mESCs with scramble (white), lincCC1 (dark gray), or lincCC2 (light gray) targeting gRNAs. Technical replicates for two different biological replicates represented as either triangles or circles. Significance of comparisons is indicated as NS p > 0.05, ∗p < 0.05, ∗∗p < 0.01.