Extended Data Table 1.
Target Gene | Direction | sgRNA | PAM site | PAM site mutation in targeting vector |
---|---|---|---|---|
hHES7 | Antisense | ACCTGCTCGCCCGGACGCCC | GGG | GGT |
mHes7 | Antisense | TAAGGAGGCACCCAAGCTAC | AGG | AAG |
hMESP2 | Antisense | GTCTCCAAAACGCGGGCGGT | GGG | GGT |