Figure 6.
Analysis of cis-regulatory elements of the eat-4 locus. (A) Overview of the eat-4 locus. Schematic of eat-4 gfp reporter constructs and their expression in hermaphrodite and male nervous systems. The constructs are referred to the eat-4 locus to scale. Gray boxed reporters have not been published before, while the information for the other constructs had been partially shown in Serrano-Saiz et al. (2013), but only in the context of the hermaphrodite and not in the male nervous system (Table S1). The red cross indicates the position of the deletion in each construct. Within the matrix, the gray intensity of each box indicates relative GFP intensity; white boxes indicate no expression. (*) CP1-6 expression was not determined. (B) Representative images of several constructs in the adult male tail. In the images for eat-4prom2 and eat-4prom2Δ7, the eat-4 fosmid (otIs518) is also shown in red as a landmark. Usually two or three lines were scored for each reporter and > 10 animals/line. Bar, 50 μm.