Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Rattus Norvegicus) | Apph | Tambini et al., 2019. Aging Cell 18: e13033 | Rat App allele with humanize Aβ region | |
| Genetic reagent (Rattus Norvegicus) | Trem2R47H | Tambini and D'Adamio, 2020 Sci Rep 10: 4122 | Rat Trem2 allele with R47H mutation | |
| Commercial assay or kit | V-PLEX Plus Aβ Peptide Panel 1 |
Meso Scale Discovery | Cat# K15200G | Used following manufacturer’s recommendations |
| Commercial assay or kit | V-PLEX Proinflammatory Panel 2 | Meso Scale Discovery | Cat# K15059D | Used following manufacturer’s recommendations |
| Commercial assay or kit | CD11b/c (Microglia) Micro-Beads, rat antibody Cat# 130-105-634 | Miltenyi Biotec | RRID:AB_2783886 | Used following manufacturer’s recommendations |
| Commercial assay or kit | Adult Brain Dissociation Kit | Miltenyi Biotec | Cat# 130-107-677 | Used following manufacturer’s recommendations |
| Commercial assay or kit | RNeasy RNA Isolation kit |
Qiagen | Cat# 74106 | Used following manufacturer’s recommendations |
| Commercial assay or kit |
High-Capacity cDNA RT kit | Thermo | Cat# 4368814) | Used following manufacturer’s recommendations |
| Commercial assay or kit | TaqMan Fast Advanced Mix |
Thermo | Cat# 4444556 | Used following manufacturer’s recommendations |
| Commercial assay or kit | Gapdh Real-Time PCR | Thermo | Rn01775763_g1 | Used following manufacturer’s recommendations |
| Commercial assay or kit | Treml1 Real-Time PCR | Thermo | Rn01511908_g1 | Used following manufacturer’s recommendations |
| Antibody | Polyclonal Goat IgG anti-Rat TNFα Cat# AF-510-NA | R and D Systems | RRID:AB_354511 | 10 ng/ml in ACSF |
| Antibody | Polyclonal Goat IgG. antibody Cat# AB-108-C | R and D Systems | RRID:AB_354267 | 10 ng/ml in ACSF |
| Software, algorithm | LinRegPCR software | hartfaalcentrum.nl | ||
| Software, algorithm | pCLAMP10 software | Molecular Devices, | ||
| Software, algorithm | Image Lab software | Biorad | RRID:SCR_014210 | |
| Software, algorithm | GraphPad Prism | RRID:SCR_002798 |