Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (mouse) | Nphp3 | NCBI | NM_028721 | |
| Cell line (human) | HEK293 | ATCC | CRL-1573 | |
| Cell line (human) | HEK-TM | Wachten et al., 2006 | ||
| Cell line (mouse) | mIMCD-3 | ATCC | CRL-2123 | |
| Transfected construct (mouse) | See Sup. Table 1 | See Sup. Table 1 | See Sup. Table 1 | |
| Biological sample (Danio rerio) | nacre | Lister et al., 1999 | ||
| Biological sample (Danio rerio) | b-actin:arl13b-gfp | Borovina et al., 2010 | ||
| Biological sample (Danio rerio) | Ubi:zebrabow | Pan et al., 2013 | ||
| Antibody (mouse) | anti-acetylated-Tubulin (mouse, monoclonal) | Sigma Aldrich | T6793 | 1:600 |
| Antibody (rabbit) | anti-GFP (rabbit, polyclonal) | Abcam | ab6556 | 1:500 |
| Antibody (rabbit) | anti-Arl13B (rabbit, polyclonal) | Proteintech | 17711–1-AP | 1:500 |
| Antibody (mouse) | anti-Arl13B (mouse, monoclonal) | Abcam | ab136648 | 1:500 |
| Antibody (donkey) | anti-mouse-Cy5 (donkey) | Dianova | 715-175-151 | 1:500 |
| Antibody (goat) | anti-rabbit-Alexa488 (goat) | Life Technologies | A11034 | 1:500 |
| Recombinant DNA reagent | cADDis cAMP assay kit | Montana Molecular | #D0201/11G | |
| Sequence-based reagent (IDT) | VHHLaM-2, VHHLaM-4 | Gene blocks (IDT) Fridy et al., 2014 | ||
| Sequence-based reagent (Ploegh lab) | VHHEnhancer | Hidde Ploegh, Boston, USA Kirchhofer et al., 2010 | ||
| Commercial assay or kit (Thermo) | Lipofectamine 2000 | Thermo Fisher Scientific | #11668030 | |
| Commercial assay or kit (Molecular Devices) | CatchPoint assay | Molecular Devices | ||
| Commercial assay or kit (Thermo) | Pierce BCA Protein Assay Kit | Thermo Fisher Scientific | 23227 | |
| Commercial assay or kit (Qiagen) | QIAquick PCR purification kit | Qiagen | #28104 | |
| Commercial assay or kit (Thermo) | mMessage mMachine T7 kit | Thermo Fisher Scientific | #AM1344 | |
| Chemical compound, drug (Sigma) | PEI | Sigma Aldrich | #64604–1G | |
| Chemical compound, drug (Merck) | ChemiBLOCKER | Merck Millipore | #2170 | |
| Chemical compound, drug (Polysciences) | Aqua-Poly/Mount | Polysciences | #18606–20 | |
| Chemical compound, drug (Thermo) | DAPI | Thermo Fisher Scientific | D1306 | 1:10.000 |
| Chemical compound, drug (Enzo) | FluoForte | Enzo Life Sciences | ENZ-52015 | |
| Chemical compound, drug (Sigma) | Fluo4-AM | Sigma Aldrich | 93596 | |
| Chemical compound, drug (Thermo) | probenecid | Thermo Fisher Scientific | P36400 | |
| Chemical compound, drug (Sigma) | NKH477 | Sigma Aldrich | N3290 | |
| Chemical compound, drug (Sigma) | Forskolin | Sigma Aldrich | F3917 | |
| Chemical compound, drug (Sigma) | IBMX | Sigma Aldrich | I5879 | |
| Software, algorithm (this paper) | CiliaQ | This publication |
https://github.com/hansenjn/CiliaQ; Hansen, 2020; copy archived at https://github.com/elifesciences-publications/CiliaQ |