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. Author manuscript; available in PMC: 2020 Jul 8.
Published in final edited form as: Cell. 2017 Jul 13;170(2):298–311.e20. doi: 10.1016/j.cell.2017.06.038

Figure 7. Altered translation kinetics of Ssb bound ribosomes.

Figure 7

(A) Average ribosome densities in translatomes and Ssb-bound translatomes related to Ssb binding. 95% CI is shaded. (B) Change in translation speed for Ssb bound and unbound ribosomes in wt and ssb1Δssb2Δ translatomes (Wilcoxon rank-sum test, p <0.0001 for all thresholds). Error bars show 95% CI. (C) Contribution of Ssb binding and mRNA features to faster translation. Error bars show 95% CI. (D) Enrichment of fast codons and depletion of slow codons in bound vs. unbound segments for indicated thresholds (Wilcoxon signed-rank test, p <0.05 for (P95, P5), p <0.0001 for other thresholds). Error bars show 95% CI. (E) Change in DMS reactivity of bound vs. unbound segments reflecting the probability of secondary structure formation with the indicated offsets from each nucleotide. Differences are significant up to an offset of 15 nt (paired t-test, p < 0.0001). See also Figures S6 and S7.