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. 2020 Jun 5;9(6):1410. doi: 10.3390/cells9061410

Figure 3.

Figure 3

(A) mRNA expression levels of p21 and p53 genes in HepG2 cells treated with 250 µM probenecid for 48 h and in Abcc6-knockdown HepG2 cells. Data are shown as fold increase compared with DMSO-treated cells calibrator for HepG2 probenecid treated cells (Probenecid) or scrambled HepG2 cells (scr-shRNA) calibrator for Abcc6-shRNA HepG2 cells. (B) Flow cytometry analysis of cell cycle phase distribution of HepG2 cells treated with 250 µM probenecid for 24 and 48 h. Data are expressed as means ± standard error (SE) of three replicates from three independent experiments. ** p < 0.01 Abcc6-shRNA vs. scr-shRNA.