Table 1.
List of antibodies used in this study.
| Antibody | Specificity | Dilution | Source |
|---|---|---|---|
| Primary Antibodies | |||
| Mouse anti-cluster differentiation 68 (ED1) | Rat macrophages | 1:200 | Serotec, Kidlington, UK |
| Mouse anti-glial fibrillary acidic protein (GFAP) | Astrocyte | 1:400 | Sigma, Poole, U |
| Mouse anti-growth associated protein-43 (GAP-43) | Regenerating axons | 1:500 | Invitrogen, Paisley, UK |
| Mouse anti-myelin associated glycoprotein (MAG) | MAG | 1:400 | Abcam, Cambridge, UK |
| Mouse anti-chondroitin sulphate proteoglycans (CSPG) | CSPG | 1:500 | Abcam, Cambridge, UK |
| Rabbit anti-laminin | Basal lamina/glia limitans and blood vessels | 1:500 | Sigma, Poole, UK |
| Rabbit anti-p75 neurotrophic receptor | Low affinity neurotrophin receptor/Schwann cells | 1:400 | Sigma, Poole, UK |
| Rabbit anti-neurite outgrowth inhibitor A (Nogo-A) | Nogo-A | 1:400 | Abcam, Cambridge, UK |
| Sheep anti-Nogo-C | Nogo-C | 1:500 | R&D systems Europe, Oxford, UK |
| Secondary Antibodies | |||
| Alexa 488 anti-mouse | Mouse IgG | 1:400 | Invitrogen, Paisley, UK |
| Texas Red anti-mouse | Mouse IgG | 1:400 | Invitrogen, Paisley, UK |
| Alexa 488 anti-rabbit | Rabbit IgG | 1:400 | Invitrogen, Paisley, UK |
| Texas Red anti-rabbit | Rabbit IgG | 1:400 | Invitrogen, Paisley, UK |
| HRP-labelled anti-rat | Rat IgG | 1:400 | Vector Laboratories, Peterborough, UK |
| HRP-labelled anti-rabbit | Rabbit IgG | 1:400 | Vector Laboratories, Peterborough, UK |
| HRP-labelled anti-sheep | Sheep IgG | 1:400 | Invitrogen, Paisley, UK |