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. 2020 May 30;12(6):1611. doi: 10.3390/nu12061611

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The inhibitory effects of eugenol and Lactobacillus plantarum ZS2058 (ZS2058) on Salmonella invasiveness and virulence gene expression in an HT-29 cell model. Effects of ZS2058 and eugenol on adhesion ability (A), invasion ability (B) of ST, and the invasion/adhesion ratio (C) in the HT-29 cell model. (D) The expression of the virulence genes InvA, AvrA, SsrB, HilA, and SopD by Polymerase Chain Reaction. (E) Grayscale analysis statistics of the electrophoretogram. To evaluate the inhibitory effect of ZS2058 and eugenol on the virulence of ST, the HT-29 cells were co-cultured with regular ST (C), eugenol-pre-treated ST (E), ZS2058 and regular ST (Z), and ZS2058 and eugenol-pre-treated ST (ZE); ST, Salmonella Typhimurium SL1344; ZS2058, Lactobacillus plantarum ZS2058. Data are expressed as means ± SEMs, n = 3. Labelled means without a common letter are significantly different, p < 0.05.