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. 2020 Jun 11;12(6):1749. doi: 10.3390/nu12061749

Figure 4.

Figure 4

Suppressive effects of OC treatments on the activity of COX1/2 levels in lung can cencer (A) A549 cells and (B) NCI-H322M cells. Cells were plated at 1 × 106 cells/100 mm culture plates in RPMI-1640 media supplemented with 10% FBS and allowed to adhere overnight. Cells were then washed twice with PBS and starved in control or OC treatments in media containing 40 ng/mL HGF for 48 h. Cells were then homogenized and lysed and the assay experiments followed the manufacturer protocol (Cayman Chemicals). SC-560 used as a COX-1 standard inhibitor while DuP-697 used as a standard COX-2 inhibitor according to the manufacturer protocol. Vertical bars in the graph indicate the % inhibition of COX1/2 ± SD. a p < 0.05 significant variation of OC treatment effects on COX1 versus COX2 activity at the same doses. b p < 0.05 significant variation between vehicle control and OC treatment effects on COX1 inhibition. c p < 0.05 significant variation between vehicle control and OC treatment effects on COX2 inhibition.