Table 1.
Contribution of amino acid side chains and stereochemistry of the HCMV pUL53 hook peptide to its interaction with pUL50.
| Alanine Scan | D-amino Acid Scan | ||
|---|---|---|---|
| Position | IC50 [µM] * ± SD | Position | IC50 [µM] * ± SD |
| WT | 0.11 ± 0.04 | WT | 0.05 ± 0.02 |
| L59 | 0.89 ± 0.12 | L59 | 0.09 ± 0.01 |
| T60 | 0.14 ± 0.02 | T60 | 0.20 ± 0.06 |
| L61 | 0.47 ± 0.01 | L61 | 0.17 ± 0.03 |
| H62 | 0.13 ± 0.02 | H62 | 0.07 ± 0.01 |
| D63 | 0.45 ± 0.01 | D63 | 0.54 ± 0.06 |
| L64 | >10 | L64 | 1.27 ± 0.31 |
| H65 | 0.42 ± 0.01 | H65 | 0.11 ± 0.01 |
| D66 | 0.12 ± 0.001 | D66 | 0.09 ± 0.01 |
| I67 | 1.30 ± 0.01 | I67 | 2.60 ± 0.06 |
| F68 | >10 | F68 | 0.11 ± 0.03 |
| R69 | 0.15 ± 0.01 | R69 | 0.56 ± 0.03 |
| E70 | 0.11 ± 0.01 | E70 | 0.08 ± 0.004 |
| H71 | 0.16 ± 0.03 | H71 | 6.51 ± 0.10 |
| P72 | 0.32 ± 0.02 | P72 | 4.00 ± 0.22 |
| E73 | 0.22 ±0.002 | E73 | 0.14 ± 0.02 |
| L74 | 1.87 ± 0.07 | L74 | 0.75 ± 0.05 |
| E75 | >10 | E75 | >10 |
| L76 | 0.1 ± 0.003 | L76 | 0.11 ± 0.01 |
| K77 | 0.37 ± 0.05 | K77 | 3.80 ± 1.12 |
| Y78 | >10 | Y78 | >10 |
| L79 | >10 | L79 | 0.94 ± 0.05 |
| N80 | 0.12 ± 0.01 | N80 | 0.20 ±0.007 |
| M81 | 1.31 ± 0.13 | M81 | 2.37 ± 0.13 |
| M82 | >10 | M82 | >10 |
| K83 | 0.22 ± 0.001 | K83 | 0.10 ± 0.02 |
| M84 | 0.22 ± 0.06 | M84 | 4.61 ± 0.12 |
| A85 | 0.11 ± 0.04 | A85 | >10 |
| I86 | 0.53 ± 0.16 | I86 | 0.86 ± 0.08 |
| T87 | 0.16 ± 0.05 | T87 | 0.11 ± 0.01 |
* Inhibition of pUL50–pUL53 interaction; WT, unaltered wild-type sequence.