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. 2020 Jun 18;10(6):925. doi: 10.3390/biom10060925

Figure 1.

Figure 1

Purification of a cancer stem cell (CSC) inhibitor derived from Cynanchum auriculatum and assessment of extracts using a mammosphere formation assay. (A) Isolation procedure for the mammosphere inhibitor. (B) Inhibition of mammospheres using high performance liquid chromatography (HPLC)-purified samples. MDA-MB-231 cells were treated with HPLC extracts or dimethyl sulfoxide (DMSO) in CSC culture medium for 7 days. Images show representative mammospheres and were obtained by microscopy (scale bar: 100 μm). (C) HPLC chromatogram of the inhibitor isolated from Cynanchum auriculatum.