Figure 5.

Pyrimethamine suppresses tumor progression in vivo. (a–g), Xenograft experiment for evaluating the in vivo tumor-suppressive effect of pyrimethamine. Pyrimethamine (20 mg/kg/day) and Taxol (15 mg/kg/day) were intraperitoneally injected, and the tumor sizes (b) and body weights (d) were measured during the experimental period. After sacrifice, the mice and the excised tumors were photographed (a), and the tumors were weighed (c). The concentration of pyrimethamine (e) and the levels of endogenous DX2 and AIMP2 proteins (f,g) in the excised tumors were determined by LC-MS/MS and Western blot (WB) analysis, respectively. Average of the quantified values among tumors were presented as a bar graph (g). Control means the carrier without chemicals. Student’s two-tailed t-test was performed for statistical analysis (* p < 0.05, ** p < 0.01). The error bars denote the standard deviation (S.D.), and the data are shown as the mean ± S.Ds.