Figure 5.

Roles of the mitogen-activated protein (MAP) kinase cascades in the upregulation of BDNF by taurine and ginsenoside Rf (g-Rf). (A) SH-SY5Y cells were treated with a combination of taurine and g-Rf for the indicated time periods, and whole-cell lysates were subjected to western blotting. (B) SH-SY5Y cells pretreated with vehicle (DMSO), SB203580, PD98059, or SP600125 for 1 h were incubated with or without a combination of taurine and g-Rf for 24 h, and whole-cell lysates were subjected to western blotting. α-Tubulin was adopted as a loading control.