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. 2020 May 26;8(6):802. doi: 10.3390/microorganisms8060802

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Bacterial translocation following NAP treatment of C. jejuni infected secondary abiotic IL10^−/− mice. On day (d) 0 and d1 secondary abiotic IL10^−/− mice were perorally infected with C. jejuni strain 81-176 by gavage and treated intraperitoneally with either synthetic NAP (open bars) or placebo (PLC; closed bars) from d2 until d5. On d6 post-infection, the abundance of C. jejuni was assessed in ex vivo biopsies derived from (A) mesenteric lymph nodes (MLN), (B) liver, (C) kidney, (D) lung, (E) spleen, and (F) cardiac blood by culture. Naive mice served as negative controls. The cumulative relative translocation rate of viable pathogens into the respective compartment out of four independent experiments (in %) as well as the numbers of culture-positive mice out of the total number of animals (in parentheses) are indicated.