Table 1. Summary statistics of the 17 microsatellite markers selected for Harbour seals (Phoca vitulina).
Sample sizes per locus (S). Number of alleles (A). Expected (He), unbiased Nei’s95 expected (H.n.b) and observed (HO) heterozygosity. Hardy–Weinberg equilibrium (HWE) p-values (P) with the standard error in parentheses. Polymorphism information content (PIC). Probability of identity (PI). Probability of parentage exclusion (PE1, single parent; PE2, a second parent given a first parent assigned; PE3, a pair of parents). Null allele frequency (Fnull). Number of repeated genotypes (Nrep and percentage (%) of the total number of individuals genotyped for each loci). Genotyping error rate per allele, E1 referring to allelic dropout rate and E2 to the false allele rate, and the 95% confidence interval (CI). Significant values are highlighted in bold (P < 0.05) for heterozygote excess.
| Genotyping error rate | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Locus | S | A | He | H.n.b | Ho | P | PIC | PI | PE1 | PE2 | PE3 | Fnull | Nrep(%) | E1 (CI 95%) | E2 (CI 95%) |
| SGPv9 | 85 | 3 | 0.277 | 0.279 | 0.329 | 0.254 (0.00038) | 0.242 | 0.558 | 0.038 | 0.123 | 0.205 | 0.023 | 10 (12) | 0.00 (0.00–0.41) | 0.00 (0.00–0.07) |
| SGPV11 | 92 | 3 | 0.275 | 0.276 | 0.174 | 0.002 (0.00004) | 0.240 | 0.561 | 0.038 | 0.122 | 0.203 | 0.107 | 10 (11) | 0.00 (−0.00–0.78) | 0.00 (0.00–0.07) |
| SGPV17 | 92 | 3 | 0.409 | 0.411 | 0.413 | 0.019 (0.00013) | 0.345 | 0.413 | 0.084 | 0.184 | 0.292 | 0.039 | 10 (11) | 0.00 (−0.00–0.20) | 0.00 (−0.00–0.07) |
| SGPV10 | 90 | 2 | 0.231 | 0.232 | 0.267 | 0.352 (0.00047) | 0.204 | 0.618 | 0.027 | 0.102 | 0.174 | 0.026 | 10 (11) | 0.00 (0.00–0.40) | 0.00 (−0.00–0.07) |
| SGPV16 | 74 | 14 | 0.886 | 0.892 | 0.919 | 0.659 (0.00033) | 0.876 | 0.023 | 0.628 | 0.772 | 0.921 | 0.013 | 10 (14) | 0.00 (0.00–0.08) | 0.00 (−0.00–0.07) |
| PVC19 | 93 | 2 | 0.350 | 0.352 | 0.344 | 1.000 (0.00000) | 0.289 | 0.484 | 0.061 | 0.144 | 0.229 | 0.039 | 10 (11) | 0.00 (−0.00–0.19) | 0.00 (0.00–0.07) |
| PVC30 | 82 | 4 | 0.493 | 0.496 | 0.476 | 0.767 (0.00037) | 0.388 | 0.362 | 0.122 | 0.204 | 0.310 | 0.039 | 10 (12) | 0.00 (−0.00–0.21) | 0.00 (0.00–0.07) |
| PVC78 | 93 | 4 | 0.243 | 0.245 | 0.204 | 0.003 (0.00005) | 0.219 | 0.597 | 0.030 | 0.113 | 0.193 | 0.058 | 10 (11) | 0.00 (−0.00–0.19) | 0.00 (0.00–0.07) |
| GS7 | 93 | 2 | 0.157 | 0.158 | 0.172 | 1.000 (0.00000) | 0.145 | 0.723 | 0.012 | 0.072 | 0.129 | 0.033 | 10 (11) | 0.00 (−0.00–0.71) | 0.00 (0.00–0.07) |
| GS2 | 91 | 3 | 0.173 | 0.174 | 0.165 | 0.557 (0.00049) | 0.163 | 0.694 | 0.015 | 0.086 | 0.156 | 0.047 | 10 (11) | 0.00 (−0.00–0.25) | 0.00 (0.00–0.07) |
| GS3 | 93 | 4 | 0.560 | 0.563 | 0.570 | 0.579 (0.00048) | 0.491 | 0.262 | 0.158 | 0.294 | 0.442 | 0.022 | 10 (11) | 0.00 (−0.00–0.16) | 0.00 (0.00–0.07) |
| H12 | 93 | 5 | 0.614 | 0.617 | 0.613 | 0.901 (0.00025) | 0.550 | 0.213 | 0.194 | 0.345 | 0.504 | 0.029 | 10 (11) | 0.00 (−0.00–0.09) | 0.00 (0.00–0.07) |
| HL20 | 90 | 2 | 0.043 | 0.044 | 0.044 | 1.000 (0.00000) | 0.043 | 0.916 | 0.001 | 0.021 | 0.041 | 0.050 | 9 (10) | 0.70 (−0.03-1.81) | 0.00 (−0.00–0.14) |
| HL15 | 93 | 3 | 0.242 | 0.243 | 0.237 | 0.707 (0.00043) | 0.215 | 0.601 | 0.029 | 0.109 | 0.186 | 0.042 | 10 (11) | 0.00 (−0.00–0.76) | 0.00 (0.00–0.07) |
| OrrFCB2 | 90 | 4 | 0.547 | 0.550 | 0.633 | 0.070 (0.00022) | 0.489 | 0.263 | 0.151 | 0.296 | 0.449 | 0.016 | 10 (11) | 0.00 (−0.00–0.11) | 0.00 (−0.00–0.07) |
| OrrFCB1 | 79 | 2 | 0.013 | 0.013 | 0.013 | 1.000 (0.00000) | 0.012 | 0.927 | 0.001 | 0.019 | 0.037 | 0.055 | 10 (13) | 0.70 (−0.03-1.81) | 0.00 (0.00–0.12) |
| OrrFCB24 | 90 | 3 | 0.510 | 0.513 | 0.956 | 0.000 (0.00000) | 0.391 | 0.359 | 0.130 | 0.202 | 0.303 | 0.009 | 9 (10) | 0.00 (0.00–0.08) | 0.00 (−0.00–0.08) |
| Mean | 89 | 3.7 | 0.354 | 0.356 | 0.384 | 0.522 (0.000) | 0.312 | ||||||||