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[Preprint]. 2020 Jul 10:2020.07.08.20147371. [Version 1] doi: 10.1101/2020.07.08.20147371

D614G Spike Variant Does Not Alter IgG, IgM, or IgA Spike Seroassay Performance

Carleen Klumpp-Thomas, Heather Kalish, Jennifer Hicks, Jennifer Mehalko, Matthew Drew, Matthew J Memoli, Matthew D Hall, Dominic Esposito, Kaitlyn Sadtler
PMCID: PMC7359543  PMID: 32676618

Abstract

Emergence of a new variant of spike protein (D614G) with increased infectivity and transmissibility has prompted many to analyze the potential role of this variant in the SARS-CoV-2 pandemic. When a new variant emerges, there is a concern regarding whether an individual exposed to one variant of a virus will have cross-reactive immune memory to the second variant. Accordingly, we analyzed the serologic reactivity of D614 (original) and G614 variant spike proteins. We found that antibodies from a high-incidence population in New York City reacted both toward the original D614 spike and the G614 spike variant. These data suggest that patients who have been exposed to either SARS-CoV-2 variant have humoral immunity that can respond against both variants. This is an important finding both for SARS-CoV-2 disease biology and for potential antibody-based therapeutics.

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