Appendix 1—figure 1. Effect of affinity-affecting mutations, differentiation time-switch and survival probability.

(A) Plot of the affinity-affecting kernel $K_{aa}$. Only around 5% of the affinity-affecting mutations increase affinity (green part of the curve for $\mathrm{\Delta }ϵ<0$). (B) Probability of MC ${\mu }_{MC}$ and PC ${\mu }_{PC}$ differentiation as a function of time from Ag injection ($t$ in days). (C) Probability $P_{\text{Ag}}(ϵ)$ that a B-cell survives Ag-binding selection process as a function of its binding energy $ϵ$, see Equation 19. On the x-axis we mark the threshold energy ${ϵ}_{\text{Ag}}$. (D) Probability $P_{\text{T}}(ϵ|\overline{ϵ})$ that a B-cell survives the T-cell selection process as a function of its binding energy $ϵ$, see Equation 20. On the x-axis we mark the threshold binding energy $\overline{ϵ}$, which depends on the binding energy distribution of the rest of the B-cell population, see Equation 20.