Correction to: Scientific Reports https://doi.org/10.1038/s41598-020-60622-1, published online 26 February 2020
The Article contains several typographical errors and omitted References in the Materials and Methods section under the subheading ‘PCR amplification and sequence analyses of 16 S rRNA, 18S rRNA, 23S rRNA and tufA’ where:
“PCR amplification and sequence analyses of 16 S rRNA, 18S rRNA, 23S rRNA and tufa
Molecular confirmation of isolates was performed via next generation sequencing of 16S/18S/23S rRNA and tufA marker regions. Genomic DNA from different mixed microalgae culture samples was isolated and high-throughput sequencing analysis was applied to each sample. Targeted amplicon libraries were constructed with universal V4 region primers [515 f (F), 5′-GTGCCAGCMGCCGCGGTAA-3′ and 806r (R), 5′-GGACTACHVHHHTWTCTAAT-3′] for 16S, TAReuk454FWD1 (F), 5′-CCAGCASCYGCGGTAATTC-3′ and TAReukREV3 (R), 5′-ACTTTCGTTCTTGATYRA-3′ primers for 18S rDNA, p23SrV_f1 (F), 5′-GGACAGAAAGACCCTATGAA-3′ and p23SrV_r1 (R), 5′-TCAGCCTGT-TATCCCTAGAG-3′ primers for 23S rDNA, (F) 5′-TGAAACAGAAMAWCGTCATT-3′ and (R) 5′-CCTTCNCGAATMGCRAAW-3′ primers for elongation factor tufA. Purified-amplicon libraries were sequenced using an Illumina MiSeq platform (2 × 300 paired-end reads).”
should read:
“PCR amplification and sequence analyses of 16S rRNA, 18S rRNA, 23S rRNA and tufa
Molecular confirmation of isolates was performed via next generation sequencing of 16S/18S/23S rRNA and tufA marker regions. Genomic DNA from different mixed microalgae culture samples was isolated and high-throughput sequencing analysis was applied to each sample. Targeted amplicon libraries were constructed with universal V4 region primers [515f (F), 5′-GTGYCAGCMGCCGCGGTAA-3′1 and 806r (R), 5′-GGACTACNVGGGTWTCTAAT-3′2] for 16S, TAReuk454FWD1 (F), 5′-CCAGCASCYGCGGTAATTC-3′ and TAReukREV3 (R), 5′-ACTTTCGTTCTTGATYRA-3′ primers3 for 18S rDNA, p23SrV_f1 (F), 5′-GGACAGAAAGACCCTATGAA-3′ and p23SrV_r1 (R), 5′-TCAGCCTGTTATCCCTAGAG-3′ primers4 for 23S rDNA, (F) 5′-TGAAACAGAAMAWCGTCATT-3′ and (R) 5′-CCTTCNCGAATMGCRAAW-3′ primers for elongation factor tufA. Purified-amplicon libraries were sequenced using an Illumina MiSeq platform (2 × 300 paired-end reads).”
The omitted References are listed below as References 1–4 respectively.
References
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