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. 2020 Jul 14;11(28):2793–2806. doi: 10.18632/oncotarget.27672

Figure 5. miR204-5p inhibitor blocked TL-induced downregulation of Cav-1 mRNA expression in NSCLC.

Figure 5

A549 and NCI-H460 cells were first treated for ~24 h with Opti-MEM (low-serum) medium containing miR204-5p inhibitor or scrambled control miRNA, then incubated for 20 h ± 50 nM/100 nM triptolide. Real-time RT-PCR was performed to analyze mRNA expression of (A) Cav-1 and (B) miR204-5p target genes. Values were normalized with either (A) 18S rRNA or (B) Snord44 expression and expressed as mean ± SD. n = 3. * indicates significantly different (p < 0.05 vs non-treated). **indicates significantly different (p < 0.01 vs non-TL treated). #indicates significantly different (p < 0.05 vs TL alone).