Skip to main content
. Author manuscript; available in PMC: 2020 Dec 17.
Published in final edited form as: Oncogene. 2020 Jun 17;39(29):5228–5239. doi: 10.1038/s41388-020-1359-4

Figure 6. p53 and TSP-1 inhibit Human Umbilical Vein Endothelial Cell network formation.

Figure 6.

(A) Network formation assay using conditioned media from transiently transfected H1299 cells. Network branch points were quantified and plotted as the mean of three biological replicates. Error bars represent SEM. Statistics were calculated with an unpaired t test.

(B-C) Network formation assay using conditioned media from 786-O (B) or RCC4 (C) cells with VHL, VHL with knockdown p53, or VHL and an antibody against TSP-1 under normoxia or hypoxia. Network branch points were quantified and plotted as the mean of three biological replicates. Error bars represent SEM. Statistics were calculated with an unpaired t test (*p<0.05, **p<0.01, ***p<0.001).