Table 1.

Stock solution and final well concentrations of superoxide production reaction mix reagents. The symbol “+” indicates the presence of the regent in the given reaction mix. Refer to the Superoxide Reaction Mix Sheet in the Supplemental Materials for calculating volumes of each component. Note: Nitroblue tetrazolium stock solution is to be made fresh for each assay.

Reagent	Stock Solution Concentration	Final Well Concentration	Reaction Mix 1	Reaction Mix 2	Reaction Mix 3	Reaction Mix 4
Nitroblue Tetrazolium (NBT)	2.5 mg/mL	0.8 mg/mL	+	+	+	+
phorbol 12-myristate 13-acetate (PMA)	100 µg/mL	0.5 µg/mL			+	+
Superoxide Dismutase (SOD)	300 µg/mL	30 µg/mL		+		+