Figure 3.

Effect of diphtheria toxin (DT) treatment on mouse skin. Representative images of LangDTR mouse tissues treated with (a) PBS (Control) and (b) DT (Depleted) (Days 1−16 post‐wounding) stained with antibodies against Langerin/CD207⁺ (red) and nuclear stain DAPI (blue). The lines indicate the dermal–epidermal junction. The numbers of CD207⁺ cells per mm² of epidermis in the (c) periphery, (d) adjacent region and (e) wound bed were counted at days 1, 3, 6 and 9 post‐wounding and compared against each other in both the control and depleted groups. The number of CD207⁺ cells per mm² of the dermis in the (f) periphery, (g) adjacent region and (h) wound bed were counted at days 1, 3, 6 and 9 post‐wounding and compared between control and depleted groups. Data presented as mean ± SEM from n = 8 wounds. Statistical differences between the groups over time were determined by two‐way analysis of variance with Bonferroni correction and indicated with an asterisk (*P < 0·05; **P < 0·01; ***P < 0·001; ****P < 0·0001).