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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Nature. 2020 Apr 22;582(7810):104–108. doi: 10.1038/s41586-020-2220-1

Fig 2. Antibodies to PfGARP bind to P. falciparum infected RBCs leading to activation of caspase-like proteases and DNA fragmentation.

Fig 2.

a) Ring stage 3D7 parasites were cultured for 24-30 hours in the presence of 10% pre-immune mouse sera or anti-PfGARP mouse sera generated by DNA vaccination. Representative dot plots demonstrating iRBCs with higher DNA content (mature parasites) in the upper gate, lower DNA content in the middle gate (immature and dying parasites), and uninfected RBCs in the lowest gate. b) Binding of polyclonal anti-PfGARP antibodies to the iRBCs in upper two gates and uninfected RBCs in the lowest gate. Incubation with anti-PfGARP results in activation of caspase-like proteases (assessed by Apostat staining, panel c), and DNA fragmentation (TUNEL staining, d) in parasites present in the upper gate. All data are representative of 3 independent experiments.