FIG 2.

Inhibitors of the SEC suppress the spread of HSV lytic infection. (A to C) HFF and MRC5 cells were infected with HSV (MOI = 0.025) for 8 h and then treated with vehicle, acyclovir (ACV), or 5 or 10 μM KL-1 or KL-2 for 16 h. (A) HFF cells were stained with anti-UL29 (green) and phalloidin-647 (F-actin, red). Scale bars, 100 μm (left 2 columns) and 20 μm (right two columns). (B and C) Viral yields from HFF-infected cells (B) and MRC5-infected cells (C). Data are means ± SEM of results from 4 replicates (ANOVA with Dunnett’s post hoc test). (D) Viral yields from HFF cells treated with vehicle or 5 or 10 μM KL-1 or KL-2 and infected with HSV (MOI = 3) for 12 h. Data are means ± SEM of results from 8 replicates (ANOVA with Dunnett’s post hoc test).