FIG 1.
Linearity and reportable range of assays A and B on a mixture of five Enterobacterales species (K. pneumoniae, E. coli, S. marcescens, Citrobacter freundii, and Enterobacter cloacae). (A and B) Extracted genomic DNA mixtures in a serial dilution were amplified by assay A (A) and assay B (B) with six technical replicates. (C and D) Percentages of Enterobacterales based on assay A (C) and assay B (D) based on the ddCT method relative to the log10 6 CFU/ml value (green). (E) Mean, standard deviation (SD), and bias of percent Enterobacterales calculated from assay A (C) and assay B (D).