Table 1. PCR primer sequences.
Table 1—source data 1. PCR primer sequences.
elife-56008-table1-data1.xlsx (10.6KB, xlsx)
| Target name | Primer name | Sequence (5'>3') | Description | Reference |
|---|---|---|---|---|
| AAVS1 | HR-AAVS1-F | gccgtctctctcctgagt | Primer 1# | PMID:23287722 |
| AAVS1 | HR-Puro-R | gtgggcttgtactcggtcat | ||
| AAVS1 | GFP-F | acgtaaacggccacaagttc | Primer 2# | This paper |
| AAVS1 | GFP-R | gaactccagcaggaccatgt | ||
| Actb | mActb-HR-F | ccatctacgagggctatgct | 5' junction | This paper |
| Actb | mActb-HR-R | gtgggcttgtactcggtcat | ||
| Actb | 3' Actb-puro-F | gtgtctctcactcggaaggac | 3' junction | |
| Actb | 3'inner-R | gcctaggtttctggaggagt | ||
| Actb | 5'outer-F | ccctgagtgtttcttgtggc | 5' junction | PMID:28524166 |
| Actb | 5'outer-R | tggagccgtacatgaactga | ||
| Actb | 5'inner-F | ccatctacgagggctatgct | ||
| Actb | 5'inner-R | tgaagcgcatgaactccttg | ||
| Actb | 3'outer-F | gccccgtaatgcagaagaag | 3' junction | |
| Actb | 3'outer-R | aggtagtgttagtgcaggcc | ||
| Actb | 3'inner-F | ctacgacgctgaggtcaaga | ||
| Actb | 3'inner-R | gcctaggtttctggaggagt | ||
| Cdx2 | 5'outer-F | acttggacagagaaagagcgatt | 5' junction | PMID:28524166 |
| Cdx2 | 5'outer-R | tccatgtgcaccttgaagc | ||
| Cdx2 | 5'inner-F | aacaaaggtccagtctacgcat | ||
| Cdx2 | 5'inner-R | ggccatgttatcctcctcgc | ||
| Cdx2 | 3'outer-F | gacggccccgtaatgcagaa | 3' junction | |
| Cdx2 | 3'outer-R | tagcttgcaaccagagaagatgt | ||
| Cdx2 | 3'inner-F | ctacgacgctgaggtcaaga | ||
| Cdx2 | 3'inner-R | cgacttcccttcaccatacaac |