Fig. 6.

Chaetocin, gemcitabine and lestaurtinib reduce late gene expression from HAdV-4 and HAdV-7. A549 cells were infected at an MOI of 10 of HAdV-4 or HAdV-7 for 1 h, and overlaid with medium containing vehicle or compound (chaetocin at 1 μM, gemcitabine at 0.5 μM and lestaurtinib at 5 μM). Cell lysates were collected at 24 hpi for analysis of late protein expression by immunoblot with an antibody raised to HAdV-5 capsid proteins. *The protein detected using the anti-HAdV-5 antibody, which cross-reacts the HAdV-4 and HAdV-7 capsid proteins, is most likely penton based on its size of ~60 kDa.