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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Allergy. 2020 Apr 14;75(8):10.1111/all.14297. doi: 10.1111/all.14297

Figure 2. IL-5 receptor expressed by HEBC is functional.

Figure 2.

A. B. and C. Differentiated HBEC at ALI were treated with rh-IL-5 (10ng/mL) for 15 and 60 minutes. Analyses of ERK, Akt and STAT5A phosphorylation in HBEC lysates from four different donors were performed by western blotting. For ERK and Akt, graphs indicate means ± SEM from four cultures. Data were analyzed by ANOVA followed by Holm-Sidak method (A) and ANOVA followed by Turkey test (B). C. Eosinophils (EOS) activated with rh-IL-5 were used as a positive control. D. HBEC from three different donors, were cultured at ALI and remained untreated (Mock) or were activated with rh-IL-5 (10ng/ml) for 24 h. RNA-sequencing was performed and a volcano plot demonstrating transcript levels increased (red) and decreased (blue) is shown.