Skip to main content
. 2020 Jul 22;58:102897. doi: 10.1016/j.ebiom.2020.102897

Fig. 5.

Fig 5

Analysis of PBAE-plasmid NPs based vaginal gel toxicity in pigs.

A) Representative images of IHC staining of the Flag protein expression at major organs cervix, ovary, urethra, vagina and uterus. CRISPR: PBAE-CRISPR/sgRNA NPs mMMT vaginal gel; Blank: PBAE NPs mMMT gel. Scale bars, 500 μm; B) Routine blood test of chronic toxicity experiment at 0 day and 1 weeks for every group. Quantitative analysis of white blood cell (WBC), red blood cell (RBC), Hemoglobin (HGB), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), platelet (PLT). The results are expressed as the mean ± SEM. n = 6 pigs per group. *P < 0.05 (Student's t-test); C) Blood biochemical test of toxicity experiment at 0 day and 1 week for each group. Quantitative analysis of alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total cholesterol (T-CHOL), blood urea nitrogen (BUN), Glucose (GLU). The results are expressed as the mean ± SEM. n = 6 pigs per group. *P < 0.05 (Student's t-test); D) H&E staining of cervix, ovary, urethra, vagina and uterus of pigs. SpCas9-HF1: PBAE-SpCas9-HF1NPs mMMT gel; eSpCas9: PBAE-eSpCas9 NPs mMMT gel; Blank: PBAE NPs mMMT gel, all the system contained 250ug plasmid. Scale bars, 200 μm.