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. Author manuscript; available in PMC: 2020 Jul 29.
Published in final edited form as: Biomacromolecules. 2017 May 17;18(6):1893–1907. doi: 10.1021/acs.biomac.7b00335

Figure 7.

Figure 7.

Quantification of BCR clustering in Raji B cells following perfusion of vehicle, fcHA, fcHALABL, cHAPLP, or fcSAgAPLP:LABL in the microfluidics plate. (A) Representative cell profile plots of IgM pixel intensity relative to normalized cell diameter, d/D. (B) Maximum IgM pixel intensity per cell determined from profile plots of individual cells (n=40). (C) Percent of cells positive for IgM capping (fully coalesced IgM clustering) determined using otsu thresholding (n=100–250 cells per sample). Statistical significance was determined by ANOVA followed by Tukey’s post hoc test with p<0.05 (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). Mean+ SD shown. Images were captured using the M04S plate and CellASIC Onyx Microfluidics platform on an Olympus IX81 inverted Epifluorescence microscope and analyzed in ImageJ.